Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD86 [EP1158Y] antibody

Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Sample
Rat Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
this antibody seems positively stained target cells with cytoplasmic staining pattern but also showed high background staining which is nuclear staining pattern.
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.

Abcam user community

Verified customer

Submitted Dec 04 2013

Sign up