Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD86 [EP1158Y] antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (blood vessels)
Specification
blood vessels
Permeabilization
Yes - saponine
Fixative
Formaldehyde

Other product details

Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% NGS + 0.1% Sapo
Dilution
1/100

Secondary antibody

Dilution
1/300

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Submitted Mar 24 2014

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