Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD86 antibody [EP1158Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary tumor (MMATV-PyMT))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 5 min high pressure
Permeabilization
Yes - 1mM EDTA, 0.05% Tween 20, pH 8.0
Specification
Mammary tumor (MMATV-PyMT)
Blocking step
5% goat serum +3% BSA in IF buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IF with 5% goat serum +3% BSA
Dilution
1/500

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Alex Flour 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The signal in the image (green) has been normalized based on the background of tissue stained with secondary only.

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Submitted Apr 14 2015

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