Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22958-106] to CD86
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-CD86 antibody [EPR22958-106]
See all CD86 primary antibodies
DescriptionRabbit monoclonal [EPR22958-106] to CD86
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD86 aa 1-250. The exact sequence is proprietary.
Database link: P42082
- Flow Cyt: Mouse splenocytes treated with 1ug/ml lipopolysaccharide.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab242142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
Flow cytometric analysis of Mouse splenocytes (treated with 1 ug/ml lipopolysaccharide (LPS) for 3 days) labelling CD86 with ab242142 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab242142 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242142 has not yet been referenced specifically in any publications.