Product nameAnti-CD86 antibody [EPR22958-106] - BSA and Azide free
See all CD86 primary antibodies
DescriptionRabbit monoclonal [EPR22958-106] to CD86 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Recombinant fragment within Mouse CD86 aa 1-250. The exact sequence is proprietary.
Database link: P42082
- FC: Mouse splenocytes treated with 1ug/ml lipopolysaccharide.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab263872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
Flow cytometric analysis of Mouse splenocytes (treated with 1 ug/ml lipopolysaccharide (LPS) for 3 days) labelling CD86 with ab242142 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab242142 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242142).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263872 has not yet been referenced specifically in any publications.