Product nameAnti-CD86 antibody [GL-1]
See all CD86 primary antibodies
DescriptionRat monoclonal [GL-1] to CD86
Tested applicationsSuitable for: IHC-Fr, Functional Studies, Flow Cyt, Blocking, ICC/IF, IPmore details
Species reactivityReacts with: Mouse
LPS-activated CBA/Cs Mouse splenic B cells.
- Mouse splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate
Concentration information loading...
Purification notesThis purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis.
Our Abpromise guarantee covers the use of ab119857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Blocking||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
ab119857 has been referenced in 10 publications.
- Gan J et al. Accelerated wound healing in diabetes by reprogramming the macrophages with particle-induced clustering of the mannose receptors. Biomaterials 219:119340 (2019). PubMed: 31357007
- Xu Q et al. Angiogenesis for tumor vascular normalization of Endostar on hepatoma 22 tumor-bearing mice is involved in the immune response. Oncol Lett 15:3437-3446 (2018). PubMed: 29467868
- Duan C et al. Sulfasalazine alters microglia phenotype by competing endogenous RNA effect of miR-136-5p and long non-coding RNA HOTAIR in cuprizone-induced demyelination. Biochem Pharmacol 155:110-123 (2018). PubMed: 29944870
- Zhou T et al. Microglia Polarization with M1/M2 Phenotype Changes in rd1 Mouse Model of Retinal Degeneration. Front Neuroanat 11:77 (2017). IHC ; Mouse . PubMed: 28928639
- Chang TZ et al. Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing. Biomater Sci 5:223-233 (2017). ICC/IF ; Mouse . PubMed: 27918020
- Ni W et al. Role of Erythrocyte CD47 in Intracerebral Hematoma Clearance. Stroke 47:505-11 (2016). WB, IHC-FoFr ; Mouse . PubMed: 26732568
- Edgtton KL et al. Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells. J Am Soc Nephrol 19:515-26 (2008). PubMed: 18184859
- Steptoe RJ et al. Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes 54:434-42 (2005). PubMed: 15677501
- Chung JB et al. Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness. J Immunol 171:1758-67 (2003). PubMed: 12902475
- Brasel K et al. Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures. Blood 96:3029-39 (2000). PubMed: 11049981