Overview

  • Product name

    Anti-CD86 antibody [OX48]
    See all CD86 primary antibodies
  • Description

    Mouse monoclonal [OX48] to CD86
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD86. Activated rat T cells.

  • Positive control

    • IHC-Fr: Rat spleen tissue. Flow Cyt: Lewis rat splenocytes.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab238468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • FUN1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T lymphocyte activation antigen CD86 precursor antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

Images

  • IHC image of CD134/OX40L receptor staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238468 at 5µg/ml. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®�647, 1/1000)) (shown in red) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

  • IHC image of CD86 staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238468 at 5µg/ml. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®647, 1/1000)) (shown in red) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

  • Lewis rat splenocytes stained with ab238468 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238468) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 5 µg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD45R PE antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

References

ab238468 has not yet been referenced specifically in any publications.

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