The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 0.25µg for 105-8 cells.
A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Expressed by activated B-lymphocytes and monocytes.
Flow cytometric analysis of 2-day unstimulated (left panel) and 2-day LPS activated (right pabel) C57Bl/6 splenocytes labeling CD86 with 0.25 µg ab171221 (filled histogram) with Rat IgG2b K Isotype Control Functional Grade Purified (open histogram) followed by anti-Rat IgG FITC. Total viable cells were used for analysis.