Anti-CD86 antibody [PO3.1] - Low endotoxin, Azide free (ab171221)

Overview

  • Product name
    Anti-CD86 antibody [PO3.1] - Low endotoxin, Azide free
    See all CD86 primary antibodies
  • Description
    Rat monoclonal [PO3.1] to CD86 - Low endotoxin, Azide free
  • Host species
    Rat
  • Tested applications
    Suitable for: Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • 2-day LPS activated C57Bl/6 splenocytes
  • General notes

    Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay

Properties

Applications

Our Abpromise guarantee covers the use of ab171221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 0.25µg for 105-8 cells.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity
    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

Images

  • Flow cytometric analysis of 2-day unstimulated (left panel) and 2-day LPS activated (right pabel) C57Bl/6 splenocytes labeling CD86 with 0.25 µg ab171221 (filled histogram) with Rat IgG2b K Isotype Control Functional Grade Purified (open histogram) followed by anti-Rat IgG FITC. Total viable cells were used for analysis.

References

ab171221 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab171221.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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