Key features and details
- Rat monoclonal [PO3.1] to CD86 - Low endotoxin, Azide free
- Suitable for: Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-CD86 antibody [PO3.1] - Low endotoxin, Azide free
See all CD86 primary antibodies
DescriptionRat monoclonal [PO3.1] to CD86 - Low endotoxin, Azide free
Tested applicationsSuitable for: Functional Studies, Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- 2-day LPS activated C57Bl/6 splenocytes
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab171221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.25µg for 105-8 cells.
A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
Flow cytometric analysis of 2-day unstimulated (left panel) and 2-day LPS activated (right pabel) C57Bl/6 splenocytes labeling CD86 with 0.25 µg ab171221 (filled histogram) with Rat IgG2b K Isotype Control Functional Grade Purified (open histogram) followed by anti-Rat IgG FITC. Total viable cells were used for analysis.
ab171221 has not yet been referenced specifically in any publications.