Recombinant
RabMAb

Recombinant Anti-CD89 antibody [EPR4622(2)] (ab124717)

Overview

  • Product name

    Anti-CD89 antibody [EPR4622(2)]
    See all CD89 primary antibodies
  • Description

    Rabbit monoclonal [EPR4622(2)] to CD89
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD89 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • U937, K562, Jurkat, and HL-60 cell lysates; Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4622(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

Target

  • Function

    Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production.
  • Tissue specificity

    Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD89 antibody
    • Fc alpha receptor antibody
    • Fc fragment of IgA, receptor for antibody
    • FCAR antibody
    • FCAR_HUMAN antibody
    • IgA Fc receptor antibody
    • Immunoglobulin alpha Fc receptor antibody
    see all

Images

  • ab124717, at 1/100, staining CD89 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-CD89 antibody [EPR4622(2)] (ab124717) at 1/1000 dilution

    Lane 1 : U937 cell lysates
    Lane 2 : K562 cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : HL-60 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 32 kDa

References

ab124717 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab124717.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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