Overview

  • Product name

    Anti-CD9 antibody [EM-04]
    See all CD9 primary antibodies
  • Description

    Rat monoclonal [EM-04] to CD9
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation of permeabilized murine bone marrow-derived mast cells (BMMC)

  • Positive control

    • Lysates from Mouse bone marrow-derived mast cells (BMMC)

Properties

Applications

Our Abpromise guarantee covers the use of ab82390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Use under non reducing condition. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 4 µg/ml.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Tissue specificity

    Expressed by a variety of hematopoietic and epithelial cells.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

Images

  • All lanes : Anti-CD9 antibody [EM-04] (ab82390) at 1 µg/ml

    Lane 1 : lysate from 1X106 bone marrow-derived mast cell (BMMC)
    Lane 2 : lysate from 0.5X106 bone marrow-derived mast cell (BMMC)
    Lane 3 : lysate from 0.25X106 bone marrow-derived mast cell (BMMC)
    Lane 4 : lysate from 0.125X106 bone marrow-derived mast cell (BMMC)

    Performed under non-reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Németh T  et al. Lineage-Specific Analysis of Syk Function in Autoantibody-Induced Arthritis. Front Immunol 9:555 (2018). Mouse . Read more (PubMed: 29616043) »
  • Murgai M  et al. KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nat Med 23:1176-1190 (2017). Read more (PubMed: 28920957) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (Blood film)
Specification
Blood film
Permeabilization
Yes - 0.1M PBS with 1% TritonX100
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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