Key features and details
- Rat monoclonal [EM-04] to CD9 (Biotin)
- Suitable for: Flow Cyt, ICC, WB, IP
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-CD9 antibody [EM-04] (Biotin)
See all CD9 primary antibodies
DescriptionRat monoclonal [EM-04] to CD9 (Biotin)
Tested applicationsSuitable for: Flow Cyt, ICC, WB, IPmore details
Species reactivityReacts with: Mouse
Permeabilized Mouse bone marrow derived mast cells.
The antibody was conjugated with Biotin-LC-NHS under optimum conditions. ab140227 is free of unconjugated biotin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purification notesab140227 was purified by protein A affinity chromatography before conjugation, followed by size exclusion chromatography after conjugation to >95% purity (SDS-PAGE).
Our Abpromise guarantee covers the use of ab140227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 4 µg/ml.
ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Use under non reducing condition. Detects a band of approximately 103 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
ab140227 has not yet been referenced specifically in any publications.