Recombinant
RabMAb

Recombinant Anti-CD9 antibody [EPR2949] - BSA and Azide free (ab195422)

Overview

  • Product name

    Anti-CD9 antibody [EPR2949] - BSA and Azide free
    See all CD9 primary antibodies
  • Description

    Rabbit monoclonal [EPR2949] to CD9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD9 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • HeLa membrane extract lysate (ab29547) can be used as a positive control in WB.
  • General notes

    Ab195422 is the carrier-free version of ab92726. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab195422 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Tissue specificity

    Expressed by a variety of hematopoietic and epithelial cells.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

Images

  • ab92726 (purified) at 1/20 immunoprecipitating CD9 in 10 μg HeLa (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST

    Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • Immunohistochemical staining of paraffin embedded rat spleen with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • Unpurified ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • Unpurified ab92726 showing positive staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • Unpurified ab92726 showing positive staining in Astrocytoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • Unpurified ab92726 showing positive staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92726).

  • This IHC data was generated using the same anti-CD9 antibody clone, EPR2949, in a different buffer formulation (cat# ab92726).

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

References

This product has been referenced in:

  • Kahlert C  et al. Identification of double-stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer. J Biol Chem 289:3869-75 (2014). Flow Cyt ; Human . Read more (PubMed: 24398677) »
  • Soldevilla B  et al. Tumor-derived exosomes are enriched in ?Np73, which promotes oncogenic potential in acceptor cells and correlates with patient survival. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 24067531) »
See all 3 Publications for this product

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