Anti-CD9 antibody [MEM-61] (ab2215)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)](https://www.abcam.com/ps/products/2/ab2215/Images/ab2215-194373-ab2215lung5ugmledit.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)")
Key features and details
- Mouse monoclonal [MEM-61] to CD9
- Suitable for: IHC-P, Mass Cytometry, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD9 antibody [MEM-61]
See all CD9 primary antibodies -
Description
Mouse monoclonal [MEM-61] to CD9 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Mass Cytometry, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD9. Pre-B line Nalm-6
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Positive control
- Prostate IHC-P: Human lung FFPE tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-61 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 20 µg/ml.
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| Mass Cytometry |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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IHC-P
Use a concentration of 20 µg/ml. |
|
Mass Cytometry
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)
IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)](https://www.abcam.com/ps/products/2/ab2215/Images/CD9-Primary-antibodies-ab2215-2.jpg)
Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Mass Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)](https://www.abcam.com/ps/products/2/ab2215/Images/ab2215-459632-anti-cd9-antibody-mem-61-mass-cytof-pbmc-human.jpg)
Mass Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)Mass cytometry (surface staining) analysis of human peripheral blood mononucleocytes after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab2215 has been referenced in 44 publications.
- Zhou L et al. Exosomal LncRNA LINC00659 transferred from cancer-associated fibroblasts promotes colorectal cancer cell progression via miR-342-3p/ANXA2 axis. J Transl Med 19:8 (2021). PubMed: 33407563
- Chen X et al. Exosomal miR-590-3p derived from cancer-associated fibroblasts confers radioresistance in colorectal cancer. Mol Ther Nucleic Acids 24:113-126 (2021). PubMed: 33738143
- Ji D et al. Small-sized extracellular vesicles (EVs) derived from acute myeloid leukemia bone marrow mesenchymal stem cells transfer miR-26a-5p to promote acute myeloid leukemia cell proliferation, migration, and invasion. Hum Cell 34:965-976 (2021). PubMed: 33620671
- Wu H et al. Extracellular-vesicles delivered tumor-specific sequential nanocatalysts can be used for MRI-informed nanocatalytic Therapy of hepatocellular carcinoma. Theranostics 11:64-78 (2021). PubMed: 33391461
- Berzaghi R et al. Secretion rates and protein composition of extracellular vesicles released by cancer-associated fibroblasts after radiation. J Radiat Res 62:401-413 (2021). PubMed: 33899109