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  1. Link

    cd9-antibody-mem-61-ab2215.pdf

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Cardiovascular Blood Platelets
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Anti-CD9 antibody [MEM-61] (ab2215)

  • Datasheet
  • SDS
Reviews (4)Q&A (3)References (44)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)
  • Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)
  • Mass Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)

Key features and details

  • Mouse monoclonal [MEM-61] to CD9
  • Suitable for: IHC-P, Mass Cytometry, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC APC/Cy7® Biotin FITC

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Overview

  • Product name

    Anti-CD9 antibody [MEM-61]
    See all CD9 primary antibodies
  • Description

    Mouse monoclonal [MEM-61] to CD9
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Mass Cytometry, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD9. Pre-B line Nalm-6

  • Positive control

    • Prostate IHC-P: Human lung FFPE tissue sections.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-61
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Reproduction
    • Fertilisation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Associated products

  • Alternative Versions

    • FITC Anti-CD9 antibody [MEM-61] (ab18241)
    • Alexa Fluor® 647 Anti-CD9 antibody [MEM-61] (ab187776)
    • APC/Cy7® Anti-CD9 antibody [MEM-61] (ab239306)
    • Biotin Anti-CD9 antibody [MEM-61] (ab28094)
    • APC Anti-CD9 antibody [MEM-61], prediluted (ab82389)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human CD9 protein (Tagged) (ab152262)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use a concentration of 20 µg/ml.
Mass Cytometry
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
Use a concentration of 20 µg/ml.
Mass Cytometry
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Tissue specificity

    Expressed by a variety of hematopoietic and epithelial cells.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P21926 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 928 Human
    • Omim: 143030 Human
    • SwissProt: P21926 Human
    • Unigene: 114286 Human
    • Alternative names

      • Tetraspanin 29 antibody
      • 5H9 antibody
      • 5H9 antigen antibody
      • Antigen defined by monoclonal antibody 602 29 antibody
      • Antigen defined by monoclonal antibody 60229 antibody
      • BA-2/p24 antigen antibody
      • BA2 antibody
      • BTCC 1 antibody
      • BTCC1 antibody
      • CD9 antibody
      • CD9 antigen antibody
      • CD9 antigen p24 antibody
      • CD9 molecule antibody
      • CD9_HUMAN antibody
      • Cell growth inhibiting gene 2 protein antibody
      • Cell growth-inhibiting gene 2 protein antibody
      • DRAP 27 antibody
      • DRAP27 antibody
      • GIG2 antibody
      • Growth inhibiting gene 2 protein antibody
      • Leukocyte antigen MIC3 antibody
      • MIC3 antibody
      • Motility related protein antibody
      • Motility-related protein antibody
      • MRP 1 antibody
      • MRP-1 antibody
      • MRP1 antibody
      • p24 antibody
      • p24 antigen antibody
      • Tetraspanin-29 antibody
      • Tspan 29 antibody
      • Tspan-29 antibody
      • TSPAN29 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)

      IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)
      Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)
      Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Mass Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)
      Mass Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)

      Mass cytometry (surface staining)  analysis of human peripheral blood mononucleocytes after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (44)

    Publishing research using ab2215? Please let us know so that we can cite the reference in this datasheet.

    ab2215 has been referenced in 44 publications.

    • Zhou L  et al. Exosomal LncRNA LINC00659 transferred from cancer-associated fibroblasts promotes colorectal cancer cell progression via miR-342-3p/ANXA2 axis. J Transl Med 19:8 (2021). PubMed: 33407563
    • Chen X  et al. Exosomal miR-590-3p derived from cancer-associated fibroblasts confers radioresistance in colorectal cancer. Mol Ther Nucleic Acids 24:113-126 (2021). PubMed: 33738143
    • Ji D  et al. Small-sized extracellular vesicles (EVs) derived from acute myeloid leukemia bone marrow mesenchymal stem cells transfer miR-26a-5p to promote acute myeloid leukemia cell proliferation, migration, and invasion. Hum Cell 34:965-976 (2021). PubMed: 33620671
    • Wu H  et al. Extracellular-vesicles delivered tumor-specific sequential nanocatalysts can be used for MRI-informed nanocatalytic Therapy of hepatocellular carcinoma. Theranostics 11:64-78 (2021). PubMed: 33391461
    • Berzaghi R  et al. Secretion rates and protein composition of extracellular vesicles released by cancer-associated fibroblasts after radiation. J Radiat Res 62:401-413 (2021). PubMed: 33899109
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD9 antibody [MEM-61]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 25 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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