- Datasheet
- Specific References (10)
- Protocols
Overview
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Product nameAnti-CD9 antibody [MEM-61]
See all CD9 primary antibodies -
DescriptionMouse monoclonal [MEM-61] to CD9
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Host speciesMouse
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SpecificityThis antibody reacts with human CD9 antigen.
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Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, Blocking, Functional Studiesmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue/ cell preparation (Human). (Pre-B line Nalm-6).
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Positive control
- Prostate IHC-P: Human lung FFPE tissue sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Purification notesThis product has been purified from tissue culture supernatant.
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ClonalityMonoclonal
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Clone numberMEM-61
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Myelomaunknown
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Use under non reducing condition. | |
| IHC-P | Use a concentration of 20 µg/ml. | |
| Flow Cyt | Use a concentration of 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Blocking | Use at an assay dependent concentration. PubMed: 22745469 | |
| Functional Studies | Use at an assay dependent concentration. The antibody induces FcgR-dependent platelet activation (aggregation). |
Target
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FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
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Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
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Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
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Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)](https://www.abcam.com/ps/products/2/ab2215/Images/ab2215-194373-ab2215lung5ugmledit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)](https://www.abcam.com/ps/products/2/ab2215/Images/CD9-Primary-antibodies-ab2215-2.jpg)
Flow Cytometry - Anti-CD9 antibody [MEM-61] (ab2215)Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References
This product has been referenced in:
- Shi Q et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). Read more (PubMed: 29096715) »
- Gupta KH et al. Apoptosis and Compensatory Proliferation Signaling Are Coupled by CrkI-Containing Microvesicles. Dev Cell 41:674-684.e5 (2017). ICC/IF ; Human . Read more (PubMed: 28633020) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"