Key features and details
- Rat monoclonal [MZ3] to CD9
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-CD9 antibody [MZ3]
See all CD9 primary antibodies
DescriptionRat monoclonal [MZ3] to CD9
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to CD9. Mouse B cells.
- Flow Cyt: C57BL/6 bone marrow cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab235119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.04 µg/ml.|
FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
C57BL/6 mouse bone marrow cells stained with ab235119 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse bone marrow cells were incubated for 30 min on ice in PBS 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235119) or Rat IgG2aκ (ab18450) (1x106 in 100µl at 0.04 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD11b antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235119 has not yet been referenced specifically in any publications.