Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [P1/33/2] to CD9
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-CD9 antibody [P1/33/2]
See all CD9 primary antibodies
DescriptionMouse monoclonal [P1/33/2] to CD9
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
- ICC/IF: HCT 116 cells. Flow cyt: HCT 116 cells.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab254175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 9.66 µg/ml.|
|Flow Cyt||Use a concentration of 1.006 µg/ml.|
FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling CD9 with ab254175 at 9.66µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HCT 116 cells. The nuclear counterstain is DAPI (Blue). Tubulin is detected with ab179504 anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody (Red).
Control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Negative control: Raji (PMID: 8921952).
Flow cytometric analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling CD9 with ab254175 at 1.006µg/ml (Red) compared with a mouse Isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254175 has not yet been referenced specifically in any publications.