Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)
Key features and details
- Mouse monoclonal [TS9] to CD9 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD9 antibody [TS9] - BSA and Azide free
See all CD9 primary antibodies -
Description
Mouse monoclonal [TS9] to CD9 - BSA and Azide free -
Host species
Mouse -
Specificity
Recognises the p24 antigen, a 24 kda protein.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD9. HeLa cell line
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Positive control
- WB: HeLa, A549, MCF7 and HEL cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
TS9 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
Target
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Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Images
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All lanes : Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) at 1 µg/ml
Lane 1 : Wild-type HeLa (No DTT) cell lysate
Lane 2 : CD9 knockout HeLa (No DTT) cell lysate
Lane 3 : A549 (Non-Reducing) cell lysate
Lane 4 : MCF7 (Non-Reducing) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab58989 observed at 24 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab58989 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line ab255375 (CD9 knockout cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58989 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) + HEL cell lysate at 130000 cells
Performed under non-reducing conditions.
Predicted band size: 24 kDaThis image was generated using the ascites version of the product.
Datasheets and documents
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Datasheet download
References (5)
ab58989 has been referenced in 5 publications.
- Zhao X et al. Downregulation of exosomal miR-1273a increases cisplatin resistance of non-small cell lung cancer by upregulating the expression of syndecan binding protein. Oncol Rep 44:2165-2173 (2020). PubMed: 32901857
- Li J et al. Mesenchymal stem cell exosomes reverse acute lung injury through Nrf-2/ARE and NF-?B signaling pathways. PeerJ 8:e9928 (2020). PubMed: 32999767
- Theodoraki MN et al. Circulating exosomes measure responses to therapy in head and neck cancer patients treated with cetuximab, ipilimumab, and IMRT. Oncoimmunology 8:1593805 (2019). PubMed: 31143513
- Battisti F et al. Tumor-Derived Microvesicles Modulate Antigen Cross-ProcessingviaReactive Oxygen Species-Mediated Alkalinization of Phagosomal Compartment in Dendritic Cells. Front Immunol 8:1179 (2017). PubMed: 28993771
- Harris HJ et al. Claudin association with CD81 defines hepatitis C virus entry. J Biol Chem 285:21092-102 (2010). Human . PubMed: 20375010