Anti-CD90 / Thy1 antibody [5E10] (ab123511)

Reviews (1)Q&A (2)References (2)
Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (ab123511)

    Key features and details

    • Mouse monoclonal [5E10] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    APC APC/Cy7® Biotin FITC PE PE/Cy5® PerCP/Cy5.5®

    Overview

    • Product name

      Anti-CD90 / Thy1 antibody [5E10]
      See all CD90 / Thy1 primary antibodies

    • Description

      Mouse monoclonal [5E10] to CD90 / Thy1

    • Host species

      Mouse

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human CD90/ Thy1. HEL erythroleukemia cells.

    • Positive control

      • Flow Cyt: Jurkat cells

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab123511 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 1 - 3 µg/ml.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    Use a concentration of 1 - 3 µg/ml.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (ab123511)
        Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (ab123511)

        Overlay histogram showing Jurkat cells stained with ab123511 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab123511, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.

        Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      References (2)

      Publishing research using ab123511? Please let us know so that we can cite the reference in this datasheet.

      ab123511 has been referenced in 2 publications.

      • Jia YY  et al. Effect of Optimized Concentrations of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Proliferation of Fibroblasts and Expression of Collagen: Related to Pelvic Floor Tissue Regeneration. Chin Med J (Engl) 131:2089-2096 (2018). PubMed: 30127219
      • Liu XL  et al. Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural-like cells. Mol Med Rep 17:7902-7910 (2018). PubMed: 29620288

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [5E10] - Azide free

       Poor
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
      Antigen retrieval step
      Other
      Sample
      Pig Tissue sections (Synovium (hip, knee))
      Specification
      Synovium (hip, knee)
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 30 2013

      Question

      searching for Ab
      reacts with human
      tested WB (ELISA)
      high conc, purified Ab
      unconjugated
      raised against peptide

      Read More
      Answer

      Thank you for contacting us.

      The following antibodies have been tested in WB and human samples, and fulfill most or all of your criteria.

      ab123511: 100 ug, conc = 1 mg/ml, but raised against HEL erythroleukemia cells
      https://www.abcam.com/CD90-Thy1-antibody-5E10-Azide-free-ab123511.html


      ab125215: 100 ug, conc = 0.5 mg/ml, raised against a synthetic peptide from the internal region of Human CD90 / Thy1
      https://www.abcam.com/CD90-Thy1-antibody-ab125215.html


      ab106934: 100 ug, conc = 0.5 mg/ml, raised against the synthetic peptide: C-PEHTYRSRTNFTSKY, corresponding to internal sequence amino acids 70-84 of Human CD90 / Thy1 (NP_006279.2)
      https://www.abcam.com/CD90-Thy1-antibody-ab106934.html


      ab110477: 100 ug, conc = 1 mg/ml, raised against a synthesized peptide derived from an internal region of Human CD90 / Thy1.
      https://www.abcam.com/CD90-Thy1-antibody-ab110477.html



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      No staining with ab123511, high background with ab62341.

      Read More
      Answer

      Thank you for your call today and for letting us know about the trouble with these antibodies.
      As we discussed, I've issued credit notes *** for your orders of these antibodies. The purchase prices will be refunded back to your credit card pending approval of our accounting department. If you have any questions about these refunds, please contact us.credits@abcam.com.
      Please let me know if there is anything else that we can do for you.

      Read More

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