Anti-CD90 / Thy1 antibody [AF-9] (ab23894)

Thank you for contacting Abcam.
Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
1. Positive selection (CD105, CD29, CD44, CD90)
2. Negative selection (CD45)
The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for your enquiry and interest in our products.

I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at these sites:

https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

https://www.abcam.com/cd45-antibody-ab10559.html

https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

https://www.abcam.com/cd105-antibody-105c02-ab44967.html

https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

There are some very informative and useful websites summarizing the different CD markers. You may wish to recommend to your customer for further consideration:

https://docs.abcam.com/pdf/immunology/cdantigen_poster.pdf

http://pathologyoutlines.com/cdmarkers.html

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting Abcam earlier today.
I am sorry that you were not getting any staining using ab23894 in IHC-Fr. As we discussed, I have processed a refund, and your credit note number is ********.
Please let me know if there is anything else I can help you with.

Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I have just received the following information from the laboratory that tested ab23894: "The secondary we used was a home-made goat anti mouse 1:500 in HRPO stabilizer. It is not commercially available. We do not have a specific protocol. Maybe if you can give us more information about what the customer would like to test we will be able to help." I'm sorry I cannot provide further protocol information in this instance,

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-paraffin sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please contact us again if you have any additional questions, and happy new year!