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    cd90--thy1-antibody-af-9-ab23894.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD90 / Thy1 antibody [AF-9] (ab23894)

  • Datasheet
  • SDS
Reviews (1)Q&A (6)References (19)

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Flow Cytometry - Anti-CD90 / Thy1 antibody [AF-9] (ab23894)

    Key features and details

    • Mouse monoclonal [AF-9] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
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    APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
    Protein
    Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    View more associated products

    Overview

    • Product name

      Anti-CD90 / Thy1 antibody [AF-9]
      See all CD90 / Thy1 primary antibodies
    • Description

      Mouse monoclonal [AF-9] to CD90 / Thy1
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD90/ Thy1.

    • Positive control

      • Flow Cyt: Jurkat cells. IHC-Fr: Human myometrial tissue.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      AF-9
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23894 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7070 Human
      • Omim: 188230 Human
      • SwissProt: P04216 Human
      • Unigene: 644697 Human
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - Anti-CD90 / Thy1 antibody [AF-9] (ab23894)
        Flow Cytometry - Anti-CD90 / Thy1 antibody [AF-9] (ab23894)
        Overlay histogram showing Jurkat cells stained with ab23894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23894, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (19)

      Publishing research using ab23894? Please let us know so that we can cite the reference in this datasheet.

      ab23894 has been referenced in 19 publications.

      • Liang Y  et al. Exosomal microRNA-144 from bone marrow-derived mesenchymal stem cells inhibits the progression of non-small cell lung cancer by targeting CCNE1 and CCNE2. Stem Cell Res Ther 11:87 (2020). PubMed: 32102682
      • Ying H  et al. Extracellular vesicles carrying miR-193a derived from mesenchymal stem cells impede cell proliferation, migration and invasion of colon cancer by downregulating FAK. Exp Cell Res 394:112144 (2020). PubMed: 32540398
      • Pan C  et al. Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest. Exp Ther Med 20:112 (2020). PubMed: 32989390
      • Malekshah OM  et al. Bioengineered adipose-derived stem cells for targeted enzyme-prodrug therapy of ovarian cancer intraperitoneal metastasis. J Control Release 311-312:273-287 (2019). PubMed: 31499084
      • Yu L  et al. Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2. Aging (Albany NY) 11:5300-5318 (2019). PubMed: 31386624
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-CD90 / Thy1 antibody [AF-9]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (myometrium)
      Specification
      myometrium
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Mar 19 2010

      Question

      Customer inquiry regarding 5 antibodies in the Human Mesenchymal Stromal Cell Marker panel:
      CD44 (ab6124)
      CD45 (ab10559)
      CD90 (ab23894)
      CD105 (ab44967)
      CD29 (ab52971)

      Customer wondering which antibodies are positive mesenchymal stromal cell markers and which are negative. Also wondering what flow cytometry procedure is in terms of gating and the expected color associated with each antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 29 2012

      Answer

      Thank you for contacting Abcam.
      Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
      1. Positive selection (CD105, CD29, CD44, CD90)
      2. Negative selection (CD45)
      The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      Answered on Nov 30 2012

      Question

      Hi,
      a customer of ours is interested in your ab93758. She has co-coltures of stromal mesenchymal stem cellsand T lymphocytes isolated from bone.
      She would like to detect only stromal mesenchymal stem, but not T lymphocytes. Can you please let me know if among the markers (CD44, CD45, CD90 CD29 and CD105) included within ab93758, there is some marker able to detect also T lymphocytes?

      Thanks in advance and kind regards

      Read More

      Abcam community

      Verified customer

      Asked on May 31 2012

      Answer

      Thank you for your enquiry and interest in our products.

      I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at these sites:

      https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

      https://www.abcam.com/cd45-antibody-ab10559.html

      https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

      https://www.abcam.com/cd105-antibody-105c02-ab44967.html

      https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

      There are some very informative and useful websites summarizing the different CD markers. You may wish to recommend to your customer for further consideration:

      https://docs.abcam.com/pdf/immunology/cdantigen_poster.pdf

      http://pathologyoutlines.com/cdmarkers.html

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on May 31 2012

      Question

      No signal in IHC on human eye sections.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 16 2012

      Answer

      Thank you for contacting Abcam earlier today.
      I am sorry that you were not getting any staining using ab23894 in IHC-Fr. As we discussed, I have processed a refund, and your credit note number is ********.
      Please let me know if there is anything else I can help you with.

      Read More

      Abcam Scientific Support

      Answered on Apr 16 2012

      Question

                          Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

      Read More

      Abcam community

      Verified customer

      Asked on Dec 14 2011

      Answer

      Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2011

      Question

      The researcher would like to know what secondary was used in ELISA with ab23894. Can we provide a specific protocol?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 03 2006

      Answer

      I have just received the following information from the laboratory that tested ab23894: "The secondary we used was a home-made goat anti mouse 1:500 in HRPO stabilizer. It is not commercially available. We do not have a specific protocol. Maybe if you can give us more information about what the customer would like to test we will be able to help." I'm sorry I cannot provide further protocol information in this instance,

      Read More

      Abcam Scientific Support

      Answered on Jul 04 2006

      Question

      I would use this antibody for immunohistochemistry on parrafin sections after ethanol 70? fixation: Is it possible? you only mentionned on frozen sections. Thank you best regards

      Read More

      Abcam community

      Verified customer

      Asked on Jan 04 2006

      Answer

      Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-paraffin sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please contact us again if you have any additional questions, and happy new year!

      Read More

      Abcam Scientific Support

      Answered on Jan 04 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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