Recombinant Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3132]
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3132] to CD90 / Thy1 -
Host species
Rabbit -
Specificity
This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human CD90/ Thy1 aa 50-150 (N terminal).
Database link: P04216 -
Positive control
- WB: Wild-type U-2 OS cell lysate, Human fetal brain and fetal kidney lysate. IHC-P: Human lung adenocarcinoma, cervical carcinoma, breast carcinoma, colon carcinoma, glioma, tonsil and brain tissues, human skeletal muscle vessels tissue and normal human tonsil vessels tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.57 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 18 kDa.
Can be blocked with ab134486. Observed molecular weight may vary depending on the glycosylation level of the target. |
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IHC-P | (6) |
1/100 - 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 18 kDa. Can be blocked with ab134486. Observed molecular weight may vary depending on the glycosylation level of the target. |
IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7070 Human
- Entrez Gene: 24832 Rat
- Omim: 188230 Human
- SwissProt: P04216 Human
- SwissProt: P01830 Rat
- Unigene: 644697 Human
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : Human kidney cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25-37 kDa why is the actual band size different from the predicted?Anti-THY1 antibody [EPR3132] (ab92574) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on the stroma in human colon carcinoma. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on human tonsil. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/500 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 35-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD90 / Thy1 antibody [EPR3132] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-45 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa -
ab92574 showing positive staining in Normal human tonsil vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Skeletal muscle vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab92574 has been referenced in 64 publications.
- Yang H et al. Tumor-infiltrating lymphocytes are functionally inactivated by CD90+ stromal cells and reactivated by combined Ibrutinib and Rapamycin in human pleural mesothelioma. Theranostics 12:167-185 (2022). PubMed: 34987640
- Kazemian A et al. Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells. Animals (Basel) 12:N/A (2022). PubMed: 35405788
- Al-Hejailan R et al. Decellularization of Full Heart-Optimizing the Classical Sodium-Dodecyl-Sulfate-Based Decellularization Protocol. Bioengineering (Basel) 9:N/A (2022). PubMed: 35447709
- Teunissen M et al. Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints. Stem Cell Res Ther 13:457 (2022). PubMed: 36064441
- Marangoni RG et al. Thy-1 plays a pathogenic role and is a potential biomarker for skin fibrosis in scleroderma. JCI Insight 7:N/A (2022). PubMed: 36066980