Recombinant Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
![Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-477285-anti-cd90-thy1-antibody-epr3132-western-blot-wildtype-u2-os-thy1-knockout-u2-os.jpg "Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3132]
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3132] to CD90 / Thy1 -
Host species
Rabbit -
Specificity
This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human CD90/ Thy1 aa 50-150 (N terminal).
Database link: P04216 -
Positive control
- Fetal brain, fetal kidney lysates, Human breast carcinoma, Human glioma and Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.57 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 18 kDa.
can be blocked with ab134486 |
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| IHC-P | (5) |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
| Notes |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 18 kDa. can be blocked with ab134486 |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7070 Human
- Entrez Gene: 24832 Rat
- Omim: 188230 Human
- SwissProt: P04216 Human
- SwissProt: P01830 Rat
- Unigene: 644697 Human
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/500 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 35-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD90 / Thy1 antibody [EPR3132] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-45 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107749-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107750-anti-cd90-thy1-antibody-epr3132-western-blot.jpg)
Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107748-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in Normal human tonsil vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107747-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Skeletal muscle vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107746-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107745-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107744-anti-cd90-thy1-antibody-epr3132.jpg)
OI-RD Scanning - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) -
![Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-8-benefits-of-recombinant-antibodies.png)
Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (52)
ab92574 has been referenced in 52 publications.
- Wu Y et al. Histochemical localization of putative stem cells in irreversible pulpitis. Oral Dis N/A:N/A (2021). PubMed: 33728761
- Seiffert N et al. In vitro recellularization of decellularized bovine carotid arteries using human endothelial colony forming cells. J Biol Eng 15:15 (2021). PubMed: 33882982
- McGuire JJ et al. Mesenchymal stem cell-derived interleukin-28 drives the selection of apoptosis resistant bone metastatic prostate cancer. Nat Commun 12:723 (2021). PubMed: 33526787
- Wang Y et al. miRNA-34a-5p regulates progression of neuroblastoma via modulating the Wnt/ß-catenin signaling pathway by targeting SOX4. Medicine (Baltimore) 100:e25827 (2021). PubMed: 34011046
- Chen D & Zhao H The inhibiting effects of microRNA-429 on the progression of pancreatic ductal adenocarcinoma cells by inhibiting epithelial mesenchymal transition. Am J Transl Res 13:3286-3293 (2021). PubMed: 34017500