Recombinant Anti-CD90 / Thy1 antibody [EPR3132] - BSA and Azide free (ab181885)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3132] - BSA and Azide free
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3132] to CD90 / Thy1 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type U-2 OS cell lysate, Human fetal brain and fetal kidney lysate. IHC-P: Human lung adenocarcinoma, cervical carcinoma, breast carcinoma, colon carcinoma, glioma, tonsil and brain tissues, human skeletal muscle vessels tissue and normal human tonsil vessels tissues.
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General notes
ab92574 is the carrier-free version of ab181885.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD90 / Thy1 antibody [EPR3132] - Low endotoxin, Azide free (ab221607)
- Alexa Fluor® 555 Anti-CD90 / Thy1 antibody [EPR3132] (ab307267)
- Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3132] (ab311183)
- Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3132] (ab311841)
- Alexa Fluor® 568 Anti-CD90 / Thy1 antibody [EPR3132] (ab313124)
- Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 17 kDa.
Observed molecular weight may vary depending on the glycosylation level of the target. |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 17 kDa. Observed molecular weight may vary depending on the glycosylation level of the target. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7070 Human
- Entrez Gene: 24832 Rat
- Omim: 188230 Human
- SwissProt: P04216 Human
- SwissProt: P01830 Rat
- Unigene: 644697 Human
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : Human kidney cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25-37 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Anti-THY1 antibody [EPR3132] (ab92574) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on the stroma in human colon carcinoma. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on human tonsil. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/500 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 35-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD90 / Thy1 antibody [EPR3132] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-45 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 showing positive staining in Normal human tonsil vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 showing positive staining in human Skeletal muscle vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 showing positive staining in human Cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab181885 has been referenced in 1 publication.
- Korsunsky I et al. Cross-tissue, single-cell stromal atlas identifies shared pathological fibroblast phenotypes in four chronic inflammatory diseases. Med 3:481-518.e14 (2022). PubMed: 35649411