Recombinant
RabMAb

Recombinant Anti-CD90 / Thy1 antibody [EPR3132] - Low endotoxin, Azide free (ab221607)

Overview

  • Product name

    Anti-CD90 / Thy1 antibody [EPR3132] - Low endotoxin, Azide free
    See all CD90 / Thy1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3132] to CD90 / Thy1 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human CD90/ Thy1 (UniProt ID: P04216).

  • Positive control

    • IHC-P: Fetal brain, fetal kidney lysates, Human breast carcinoma, glioma, pancreatic carcinoma and brain tissues.
  • General notes

    ab221607 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab221607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.

can be blocked with ab134486

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling CD90/Thy1 with ab221607 at 1/4000 dilution (0.26 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on stromal cells in human pancreatic carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92574 showing positive staining in Normal human tonsil vessels tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92574 showing positive staining in human Skeletal muscle vessels tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92574 showing positive staining in human Cervical carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92574 showing positive staining in human Lung adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).

    References

    ab221607 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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