Name: Anti-CD90 / Thy1 antibody [EPR3133]
Brand: RabMAb
SKU: ab133350
Rating: 5 (5 reviews)

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Primary - Rabbit Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
IHC-P, ICC/IF, WB

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)
SDS-PAGE

View more associated products

Product name

Anti-CD90 / Thy1 antibody [EPR3133]
See all CD90 / Thy1 primary antibodies
Description

Rabbit monoclonal [EPR3133] to CD90 / Thy1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues near the N terminal of Human CD90/ Thy1 (UniProt P04216).
Positive control
- Human fetal brain and Human bone marrow lysates; Human glioma and kidney tissues
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3133
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab133350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.
WB		1/1000 - 1/5000. Predicted molecular weight: 17 kDa.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P04216 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7070 Human
- Omim: 188230 Human
- SwissProt: P04216 Human
- Unigene: 644697 Human
Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
- CDw90 antibody
- FLJ33325 antibody
- MGC128895 antibody
- T25 antibody
- Theta antigen antibody
- Thy 1 antibody
- Thy 1 cell surface antigen antibody
- Thy 1 membrane glycoprotein antibody
- Thy 1 T cell antigen antibody
- Thy 1.2 antibody
- Thy-1 antigen antibody
- Thy-1 membrane glycoprotein antibody
- Thy1 antibody
- Thy1 antigen antibody
- Thy1 T cell antigen antibody
- Thy1.1 antibody
- Thy1.2 antibody
- THY1_HUMAN antibody
- Thymus cell antigen 1, theta antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Immunohistochemical analysis of paraffin-embedded Human normal kidney tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/2000 dilution

Lane 1 : Human marrow whole cell lysates
Lane 2 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 17 kDa

Exposure time: 3 minutes

Blocking/Diluting buffer 5% NFDM/TBST

Observed MW: 25-35 KDa
Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

ab133350 staining CD90 / Thy1 in the SH-SY5Y human neuroblastoma epithelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI was used as a nuclear counter stain and Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain microtubules.
Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : Fetal brain whole cell lysates
Lane 3 : Fetal kidney whole cell lysates
Lane 4 : Human spleen whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 17 kDa

Exposure time: 30 seconds

Blocking/Diluting buffer 5% NFDM/TBST

Observed MW: 25-35 KDa
Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human bone marrow lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab133350 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Immunohistochemical analysis of paraffin embedded normal Human tonsil vessels tissue using ab133350 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab133350 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

This product has been referenced in:

Spano C et al. Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer. Sci Rep 9:1788 (2019). Read more (PubMed: 30742129) »
Naldaiz-Gastesi N et al. Isolation and characterization of myogenic precursor cells from human cremaster muscle. Sci Rep 9:3454 (2019). Read more (PubMed: 30837559) »

See all 31 Publications for this product

Publishing research using ab133350? Please let us know so that we can cite the reference in this datasheet.

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1-6 of 6 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Nerve)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA 70C 2hr

Permeabilization

Specification

Nerve

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Apr 17 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Breast cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Breast cancer

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Feb 13 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Brain

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Nov 08 2017

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Sample

Human Cell (iPS-derived cardiofibroblast)

Specification

iPS-derived cardiofibroblast

Permeabilization

Yes - 0.2% triton X

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Aug 12 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (human breast cancer)

Specification

human breast cancer

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citric acid, pH 6

Permeabilization

Yes - 0.05% Tween20

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 01 2013

Question

What is the concentration of antibody ab133350?

Abcam community

Verified customer

Asked on Feb 22 2013

Answer

Thank you for contacting us.

The concentration of ab is XXXX mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Feb 22 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [EPR3133]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [EPR3133]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [EPR3133]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD90 / Thy1 antibody [EPR3133]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [EPR3133]