Recombinant Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
![Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)](https://www.abcam.com/ps/products/133/ab133350/Images/ab133350-420284-anti-cd90-thy1-antibody-epr3133-westernblot-human.jpg "Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3133] to CD90 / Thy1
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3133]
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3133] to CD90 / Thy1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Human tonsil tissue; WB: Human glioma and brain tissue lysate; ICC: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3133 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 568 Anti-CD90 / Thy1 antibody [EPR3133] (ab201848)
- Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3133] (ab202334)
- Alexa Fluor® 555 Anti-CD90 / Thy1 antibody [EPR3133] (ab202511)
- Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3133] (ab202512)
- Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
- Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
|
|
| ICC |
1/50.
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| Notes |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
|
ICC
1/50. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7070 Human
- Omim: 188230 Human
- SwissProt: P04216 Human
- Unigene: 644697 Human
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution (Purified)
Lane 1 : Human glioma lysate
Lane 2 : Human brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 25-35 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 24116172 and PMID: 30177788).
Blocking Buffer and concentration: 5% NFDM/TBST
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![Immunocytochemistry - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)](https://www.abcam.com/ps/products/133/ab133350/Images/ab133350-12-anti-cd90-thy1-antibody-epr3133-immunocytochemistry-jurkat-human.jpg)
Immunocytochemistry - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD90 / Thy1 with Purified ab133350 at 1:50 dilution (2.44 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)](https://www.abcam.com/ps/products/133/ab133350/Images/ab133350-10-anti-cd90-thy1-antibody-epr3133-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD90 / Thy1 with Purified ab133350 at 1:4000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)](https://www.abcam.com/ps/products/133/ab133350/Images/ab133350-14-benefits-of-recombinant-antibodies.png)
Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab133350 has been referenced in 51 publications.
- Liu Y et al. Isolation and characterization of mesenchymal stem cells from umbilical cord of giant panda. Tissue Cell 71:101518 (2021). PubMed: 33676235
- Chen H et al. BMP9-ID1 signaling promotes EpCAM-positive cancer stem cell properties in hepatocellular carcinoma. Mol Oncol 15:2203-2218 (2021). PubMed: 33834612
- He Y et al. Human umbilical cord-derived mesenchymal stem cells improve the function of liver in rats with acute-on-chronic liver failure via downregulating Notch and Stat1/Stat3 signaling. Stem Cell Res Ther 12:396 (2021). PubMed: 34256837
- Yuan S et al. Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR. Drug Des Devel Ther 14:5419-5430 (2020). PubMed: 33324039
- Nishida Y et al. Intra-Articular Injection of Stromal Cell-Derived Factor 1a Promotes Meniscal Healing via Macrophage and Mesenchymal Stem Cell Accumulation in a Rat Meniscal Defect Model. Int J Mol Sci 21:N/A (2020). PubMed: 32751701