Recombinant
RabMAb

Recombinant Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)

Rabbit recombinant monoclonal CD90 / Thy1 antibody [EPR3133]. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 24 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-CD90 / Thy1 antibody [EPR3133]
    See all CD90 / Thy1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3133] to CD90 / Thy1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human CD90/ Thy1 (UniProt P04216).

  • Positive control

    • Human fetal brain and Human bone marrow lysates; Human glioma and kidney tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human normal kidney tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.
    • All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/2000 dilution

      Lane 1 : Human marrow whole cell lysates
      Lane 2 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates
      Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 17 kDa


      Exposure time: 3 minutes


      Blocking/Diluting buffer 5% NFDM/TBST

      Observed MW: 25-35 KDa

    • ab133350 staining CD90 / Thy1  in the SH-SY5Y human neuroblastoma epithelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI was used as a nuclear counter stain and Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain microtubules.

    • All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
      Lane 2 : Fetal brain whole cell lysates
      Lane 3 : Fetal kidney whole cell lysates
      Lane 4 : Human spleen whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 17 kDa


      Exposure time: 30 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

      Observed MW: 25-35 KDa

    • All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human bone marrow lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab133350 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil vessels tissue using ab133350 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab133350 showing +ve staining.

    References

    This product has been referenced in:

    See all 29 Publications for this product

    Customer reviews and Q&As

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