Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3133] to CD90 / Thy1 (Alexa Fluor® 647)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAnti-CD90 / Thy1 antibody [EPR3133] (Alexa Fluor® 647)
See all CD90 / Thy1 primary antibodies
DescriptionRabbit monoclonal [EPR3133] to CD90 / Thy1 (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD90/ Thy1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P04216
- ICC/IF: K562 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
- Anti-CD90 / Thy1 antibody [EPR3133] (Alexa Fluor® 568) (ab201848)
- Anti-CD90 / Thy1 antibody [EPR3133] (Alexa Fluor® 555) (ab202511)
- Anti-CD90 / Thy1 antibody [EPR3133] (Alexa Fluor® 594) (ab202512)
- Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
- Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
Our Abpromise guarantee covers the use of ab202334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in K562 cells fixed with 100% methanol (5 min)
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
ab202334 staining CD90 / Thy1 in K562 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202334 at 1/100 dilution (shown in red). Cell membranes were labelled with WGA (Alexa Fluor® 488, shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab202334 has not yet been referenced specifically in any publications.