Product nameAnti-CD90 / Thy1 antibody [IBL-6/23]
See all CD90 / Thy1 primary antibodies
DescriptionRat monoclonal [IBL-6/23] to CD90 / Thy1
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-FoFrmore details
Species reactivityReacts with: Mouse
Mouse spleen cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab3105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 - 10 µg/ml.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use a concentration of 1 - 10 µg/ml. Fix with acetone.|
|IP||Use a concentration of 1 - 10 µg/ml.|
|IHC-FoFr||Use a concentration of 1 - 10 µg/ml.|
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
ab3105 used in Flow Cytometry.
ab3105 used at 5µg/ml to stain murine thymus cells. The secondary used was an Alexa Fluor 488 conjugated goat anti-rat IgG monoclonal, used at a 1/1000 dilution.
ab3105 staining CD90 / Thy1 in murine bone marrow cells (smeared directly onto glass slide) by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, blocked with 1% serum for 1 hour at 37°C and then incubated with ab3105 at a 1/300 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rat polyclonal used at a 1/400 dilution. Dapi stains nuclei in blue.
Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.
This product has been referenced in:
- He R et al. Link Protein N-Terminal Peptide as a Potential Stimulating Factor for Stem Cell-Based Cartilage Regeneration. Stem Cells Int 2018:3217895 (2018). Read more (PubMed: 29531532) »
- Dinh PC et al. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies. Respir Res 18:132 (2017). Flow Cyt . Read more (PubMed: 28666430) »