Overview

  • Product name
    Anti-CD90 / Thy1 antibody [IBL-6/23]
    See all CD90 / Thy1 primary antibodies
  • Description
    Rat monoclonal [IBL-6/23] to CD90 / Thy1
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 10 µg/ml.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use a concentration of 1 - 10 µg/ml. Fix with acetone.
IP Use a concentration of 1 - 10 µg/ml.
IHC-FoFr Use a concentration of 1 - 10 µg/ml.

Target

  • Function
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Images

  • ab3105 used in Flow Cytometry.
    ab3105 used at 5µg/ml to stain murine thymus cells. The secondary used was an Alexa Fluor 488 conjugated goat anti-rat IgG monoclonal, used at a 1/1000 dilution.

    See Abreview

  • ab3105 staining CD90 / Thy1 in murine bone marrow cells (smeared directly onto glass slide) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, blocked with 1% serum for 1 hour at 37°C and then incubated with ab3105 at a 1/300 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rat polyclonal used at a 1/400 dilution. Dapi stains nuclei in blue.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.

References

This product has been referenced in:
  • He R  et al. Link Protein N-Terminal Peptide as a Potential Stimulating Factor for Stem Cell-Based Cartilage Regeneration. Stem Cells Int 2018:3217895 (2018). Read more (PubMed: 29531532) »
  • Dinh PC  et al. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies. Respir Res 18:132 (2017). Flow Cyt . Read more (PubMed: 28666430) »
See all 9 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

I can confirm that ab3105 was tested on formalin-fixed paraffin-embedded tissue sections from human reactive tonsil but we didn't detect signal with this antibody.

Read More
Application
Immunohistochemistry (Frozen sections)
Blocking step
NOVOCASTRA protein block as blocking agent as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Sample
Mouse Tissue sections (Mouse skeletal muscle)
Specification
Mouse skeletal muscle
Permeabilization
Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow cells smear)
Specification
Bone marrow cells smear
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin 0.5%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Ms. Sophie Nadaud

Verified customer

Submitted Feb 23 2012

Answer

Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this. As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. The ice pack may be thawed or even at room temperature when you receive it; please be assured that this is normal and that your product is safe to use. Once you have received the vial, please follow the long-term storage instructions on the datasheet. Our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found at: https://www.abcam.com/index.html?pageconfig=contactus I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. PS: Attached is the tested data of few of our antibodies.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Thymus)
Specification
Thymus
Permeabilization
No

Abcam user community

Verified customer

Submitted Jul 14 2011

Answer

Thank you for your enquiry, we have not tested the antibody ab3105 in immunocytochemistry but the antibody is very likely to work as it works very well in perfusion fixed tissue and frozen post fixed tissue. I would definitely recommend fixing mouse cells prior to staining them, try ice cold 4% paraformaldehyde for 10,20,30min, and try also ice cold methanol 10min (or a combination of both). If your staining is still weak, make sure you have plenty of permabilising agent (tritonx100 0.3%v/v) in the antibody dilution buffer. If you still experience problems please do not hesitate to contact us again and provide us with a detailed protocol and we will investigate it into details to find out the root of the problem, I enclose a link to a form which will help you put together your protocol details easily, should you need our help: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=3105&mode=questionaire

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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