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  1. Link

    cd90--thy1-antibody-ibl-623-ab3105.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (26)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)
  • Flow Cytometry - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

Key features and details

  • Rat monoclonal [IBL-6/23] to CD90 / Thy1
  • Suitable for: IHC-P, Flow Cyt, IHC-FoFr
  • Reacts with: Mouse
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD90 / Thy1 antibody [IBL-6/23]
    See all CD90 / Thy1 primary antibodies
  • Description

    Rat monoclonal [IBL-6/23] to CD90 / Thy1
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to CD90/ Thy1.
    Database link: P01831

  • Positive control

    • Flow Cyt: mouse peripheral lymph node IHC-P: mouse spleen tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IBL-6/23
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
  • Recombinant Protein

    • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (1)
Use a concentration of 5 µg/ml.
IHC-FoFr
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 5 µg/ml.
IHC-FoFr
Use at an assay dependent concentration.

Target

  • Function

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P04216 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21838 Mouse
    • SwissProt: P01831 Mouse
    • Unigene: 3951 Mouse
    • Alternative names

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

      Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.

    • Flow Cytometry - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)
      Flow Cytometry - Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)

      Flow cytometry analysis of mouse peripheral lymph node labeling CD90 / Thy1 with ab3105 at 5 ug/mL.

      PE-conjugated anti-rat IgG.

      Red line: isotype control labeling with <3% postive events. 

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (26)

    Publishing research using ab3105? Please let us know so that we can cite the reference in this datasheet.

    ab3105 has been referenced in 26 publications.

    • Sommer K  et al. Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice. Eur J Trauma Emerg Surg N/A:N/A (2021). PubMed: 33813603
    • Storer MA  et al. Acquisition of a Unique Mesenchymal Precursor-like Blastema State Underlies Successful Adult Mammalian Digit Tip Regeneration. Dev Cell 52:509-524.e9 (2020). PubMed: 31902657
    • Chen PY  et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms. Cell Stem Cell 26:542-557.e11 (2020). PubMed: 32243809
    • Tibullo D  et al. Ixazomib Improves Bone Remodeling and Counteracts sonic Hedgehog signaling Inhibition Mediated by Myeloma Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32019102
    • Yu W  et al. Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro. Medicine (Baltimore) 99:e20628 (2020). PubMed: 32569193
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-3 of 3 Abreviews

    Immunohistochemistry (Frozen sections) abreview for Anti-CD90 / Thy1 antibody [IBL-6/23]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    NOVOCASTRA protein block as blocking agent as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Mouse skeletal muscle)
    Specification
    Mouse skeletal muscle
    Permeabilization
    Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD90 / Thy1 antibody [IBL-6/23]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bone marrow cells smear)
    Specification
    Bone marrow cells smear
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - saponin 0.5%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More

    Ms. Sophie Nadaud

    Verified customer

    Submitted Feb 23 2012

    Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [IBL-6/23]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Thymus)
    Specification
    Thymus
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 14 2011

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