Key features and details
- Mouse monoclonal [MRC OX-7] to CD90 / Thy1 - BSA and Azide free
- Suitable for: WB, IHC-Fr, IHC-P, Flow Cyt, ICC/IF, IP
- Reacts with: Rat, Horse
- Isotype: IgG1
Product nameAnti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free
See all CD90 / Thy1 primary antibodies
DescriptionMouse monoclonal [MRC OX-7] to CD90 / Thy1 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, Flow Cyt, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Horse
Predicted to work with: Mouse, Rabbit, Guinea pig
Full length protein.
- WB: rat brain tissue lysate and PC12 whole cell lysate. IF/ICC: PC12 cells.
This antibody clone is manufactured by Abcam.
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
This product is a PBS-only buffer formulated version of ab225.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
Clone numberMRC OX-7
Light chain typekappa
Our Abpromise guarantee covers the use of ab222781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 16723538|
|IHC-P||Use at an assay dependent concentration. PubMed: 23616767|
|Flow Cyt||Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
The flow cytometry data shown was generated using the same antibody clone in a different buffer formulation (ab225).
Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [B11/6](ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.
The ICC/IF data shown was generated using the same antibody clone in a different buffer formulation (ab225).
ab225 stained PC12 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab222781 has not yet been referenced specifically in any publications.