Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
Key features and details
- Mouse monoclonal [MRC OX-7] to CD90 / Thy1 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), ICC
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free
See all CD90 / Thy1 primary antibodies -
Description
Mouse monoclonal [MRC OX-7] to CD90 / Thy1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICCmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: rat brain tissue lysate and PC12 whole cell lysate. IF/ICC: PC12 cells. Flow Cyt: Rat splenocytes.
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General notes
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
ab222781 is the carrier-free version of ab225.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-7 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
Observed molecular weight may vary depending on the glycosylation level of the target. |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa). Observed molecular weight may vary depending on the glycosylation level of the target. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Flow cytometry staining of Lewis rat splenocytes with ab222781 (right) or mouse IgG1κ (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab222781) or mouse IgG1κ (ab170190) isotype (1x106 in 100 µL at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at dilution for 30 min on ice.
The cells were simultaneously stained with CD4.Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live CD3 positive T cells.
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The flow cytometry data shown was generated using the same antibody clone in a different buffer formulation (ab225).
Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [B11/6](ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.
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The ICC/IF data shown was generated using the same antibody clone in a different buffer formulation (ab225).
ab225 stained PC12 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab222781 has been referenced in 1 publication.
- Zhang S et al. Porcine pancreas mesenchymal cell characterization and functional differentiation into insulin-producing cells in vitro. Mol Med Rep 24:N/A (2021). PubMed: 34414446