Key features and details
- Mouse monoclonal [MRC OX-7] to CD90 / Thy1 (PerCP)
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Product nameAnti-CD90 / Thy1 antibody [MRC OX-7] (PerCP)
See all CD90 / Thy1 primary antibodies
DescriptionMouse monoclonal [MRC OX-7] to CD90 / Thy1 (PerCP)
ConjugationPerCP. Ex: 482nm, Em: 675nm
SpecificityRecognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
Predicted to work with: Horse
Full length protein corresponding to Rat CD90/ Thy1 aa 1 to the C-terminus.
Database link: P01830
- Flow Cyt: PC12 (NGF differentiated)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Clone numberMRC OX-7
Light chain typekappa
- Anti-CD90 / Thy1 antibody [MRC OX-7] (HRP) (ab199219)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (PE) (ab33694)
Our Abpromise guarantee covers the use of ab216354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
Overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216354, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
This antibody gave a positive signal in PC12 (NGF differentiated) cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab216354 has not yet been referenced specifically in any publications.