Overview

  • Product name
    Anti-CD90 / Thy1 antibody [MRC OX-7]
    See all CD90 / Thy1 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-7] to CD90 / Thy1
  • Host species
    Mouse
  • Specificity
    Recognizes the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.
  • Tested applications
    Suitable for: Flow Cyt, IP, WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Horse
    Predicted to work with: Mouse, Rabbit, Guinea pig
  • Immunogen

    Full length protein corresponding to Rat CD90/ Thy1.

  • Positive control
    • WB: rat brain tissue lysate and PC12 whole cell lysate. IF/ICC: PC12 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23616767
ICC/IF 1/1000.
IHC-Fr Use at an assay dependent concentration. PubMed: 16723538

Target

  • Function
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Images

  • Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [B11/6](ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence analysis of horse adipose and bone marrow stem cells labeling CD90 / Thy1 with ab225 at 1/1000 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton x100 0.1%. The cells were blocked with 10% BSA for 30 minutes at 37°C, followed by staining with ab225 at 1/1000 for 12 hours in PBS+IGEPAL+BSA+10%NGS at 4°C. Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886) was used as the secondary antibody at 1/400 dilution.

    See Abreview

  • Immunohistochemistry (Frozen sections) analysis of rat spleen tissue labeling CD90 / Thy1 with ab225 at 1/100 dilution. The tissue was fixed in paraformaldehyde  followed by blocking in 10% serum for 20 minutes. The tissue was then stained with ab225 at 1/100 in PBS for 6 hours at 4°C. A polyclonal donkey anti-mouse Alexa Fluor® 488 secondary antibody was used at 1/1000 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of rat sciatic nerve schwann cells and fibroblasts labeling CD90 / Thy1 with ab225 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100. The cells were blocked with 5% serum for 1 hour at 21°C, followed by staining with ab225 at 1/400 in 0.2% BSA for 1 hour at 37°C. A polyclonal goat anti-mouse Alexa Fluor® 546 secondary antibody was used at 1/500 dilution.

    See Abreview

  • All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225) at 5 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 35-37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Rat CD90/Thy1 is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25-37 kDa). 

  • ab225 stained PC12 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • This image was kindly supplied as part of the review submitted by Nick Voilley. Rat retinal ganglion cell labelled with ab225 as a primary antibody and an anti-mouse F(ab)' 2 coupled to Alexa488 as a secondary antibody. The cell is approximately 15 micrometers in diameter. Only the cells labelled in green in the culture bear action potentials when stimulated. These three elements (reactivity to ab225, size and elecrophysiological parameters) clearly indicate the cell is a ganglion cell.

References

This product has been referenced in:
  • Gholinejad M  et al. Adenosine decreases oxidative stress and protects H2O2-treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8:439-446 (2018). Read more (PubMed: 29732147) »
  • Li R  et al. Potential of rhBMP-2 and dexamethasone-loaded Zein/PLLA scaffolds for enhanced in vitro osteogenesis of mesenchymal stem cells. Colloids Surf B Biointerfaces 169:384-394 (2018). IF . Read more (PubMed: 29803154) »
See all 47 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Primary bone marrow-derived stromal cells)
Permeabilization
No
Specification
Primary bone marrow-derived stromal cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Egi Kardia

Verified customer

Submitted Sep 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step
Other
Sample
Rabbit Tissue sections (Bone and cartilage)
Specification
Bone and cartilage
Permeabilization
No
Fixative
70 % Ethanol

Abcam user community

Verified customer

Submitted Aug 26 2014

Application
Flow Cytometry
Sample
Mouse Cell (mMSC)
Specification
mMSC
Preparation
Cell harvesting/tissue preparation method: bone marrow extraction
Sample buffer: PBS
Fixation
Methanol
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mMSC)
Loading amount
70 µg
Specification
mMSC
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 18 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Blocking step
FBS / BSA in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: RT°C

Mr. Raul Peña

Verified customer

Submitted Dec 03 2012

Question
Answer

Thank you very much for your call yesterday and for your patience.
I have confirmed with the lab that the antibody is tested with non-permeabilized cells in flow cytometry. So, the epitope is extracellular.
I hope that this information will be useful, but if you have any further questions please let me know and I'll be happy to help.

Read More

Answer

Thank you for your enquiry and your interest.

Unfortunately, we currently do not have any data (either empirical or from literature) that would support reactivity to porcine tissue. A quick BLAST comparison shows only a 75% homology between the rodent and porcine CD90.

Based on this,we would expect only a weak reactivity to porcine tissue.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab11414andab225(different lots). The order will ship on Monday for delivery on Tuesday.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Adipose and bone marrow stem cells)
Specification
Adipose and bone marrow stem cells
Fixative
Paraformaldehyde
Permeabilization
Yes - TRITON x100 0,1%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Ms. Vega Villar

Verified customer

Submitted Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Sep 06 2011

1-10 of 15 Abreviews or Q&A

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