Overview

  • Product name

    Anti-CD90 / Thy1 antibody [MRC OX-7] (HRP)
    See all CD90 / Thy1 primary antibodies
  • Description

    Mouse monoclonal [MRC OX-7] to CD90 / Thy1 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Guinea pig
  • Immunogen

    Full length protein corresponding to Rat CD90/ Thy1.

  • Positive control

    • WB: Rat brain tissue lysate and PC12 whole cell lysate.
  • General notes

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

Properties

Applications

Our Abpromise guarantee covers the use of ab199219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Images

  • All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] (HRP) (ab199219) at 1/5000 dilution

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199219 has not yet been referenced specifically in any publications.

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