Key features and details
- Mouse monoclonal [MRC OX-7] to CD90 / Thy1 (HRP)
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Product nameAnti-CD90 / Thy1 antibody [MRC OX-7] (HRP)
See all CD90 / Thy1 primary antibodies
DescriptionMouse monoclonal [MRC OX-7] to CD90 / Thy1 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Guinea pig
Full length protein corresponding to Rat CD90/ Thy1.
- WB: Rat brain tissue lysate and PC12 whole cell lysate.
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Clone numberMRC OX-7
Light chain typekappa
- Anti-CD90 / Thy1 antibody [MRC OX-7] (PerCP) (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (PE) (ab33694)
Our Abpromise guarantee covers the use of ab199219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).|
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] (HRP) (ab199219) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab199219 has not yet been referenced specifically in any publications.