Recombinant
RabMAb

Recombinant Anti-CD94 antibody [EPR21003] (ab235441)

Overview

  • Product name

    Anti-CD94 antibody [EPR21003]
    See all CD94 primary antibodies
  • Description

    Rabbit monoclonal [EPR21003] to CD94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD94 aa 50-150. The exact sequence is proprietary.
    Database link: Q13241

  • Positive control

    • WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T-cell lymphoma tissue. IP: NK-92 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21003
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
  • Tissue specificity

    Natural killer cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 94 antibody
    • CD94 antibody
    • CD94 antigen antibody
    • Killer cell lectin like receptor subfamily D member 1 antibody
    • Killer cell lectin-like receptor subfamily D member 1 antibody
    • KLRD 1 antibody
    • KLRD1 antibody
    • KLRD1 protein antibody
    • KLRD1_HUMAN antibody
    • KP 43 antibody
    • KP43 antibody
    • Natural killer cells antigen CD94 antibody
    • NK cell receptor antibody
    • OTTHUMP00000238754 antibody
    • OTTHUMP00000238755 antibody
    • OTTHUMP00000238756 antibody
    • OTTHUMP00000238758 antibody
    • OTTHUMP00000239093 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human NK/T-cell lymphoma (PMID: 11253136, PMID: 10828054, PMID: 12816864) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-CD94 antibody [EPR21003] (ab235441) at 1/1000 dilution + NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 20 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

     

  • CD94 was immunoprecipitated from 0.35 mg NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab235441 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235441 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: NK-92 whole cell lysate 10 µg (Input).
    Lane 2: ab235441 IP in NK-92 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235441 in NK-92 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

    This blot was developed with a high sensitivity ECL substrate.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in the marginal zone of human tonsil (PMID: 11253136) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab235441 has not yet been referenced specifically in any publications.

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