Product nameAnti-CD96 antibody [BLR065G] - BSA free
See all CD96 primary antibodies
DescriptionRabbit monoclonal [BLR065G] to CD96 - BSA free
Tested applicationsSuitable for: IP, WB, IHC-P, ICCmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CD96 aa 29-502 (extracellular). The exact sequence is proprietary. NP_005807.1.
Database link: P40200-2
- IHC-P: Human ovarian carcinoma and tonsil tissues. ICC: MOLT-4 cells. WB: MOLT-4, A2058, CCRF-CEM, KG-1 and HH whole cell lysates. IP: MOLT-4 whole cell lysate.
This product is sold under License from Bethyl Laboratories, Inc
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab264416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 6 µl/mg lysate.
|WB||1/1000. Predicted molecular weight: 64 kDa.|
|IHC-P||1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
|ICC||1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FunctionMay be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Tissue specificityExpressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
Involvement in diseaseDefects in CD96 are a cause of C syndrome (CSYN) [MIM:211750]; also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. Note=A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint.
Defects in CD96 are a cause of C-like syndrome (CLSYN) [MIM:605039]; also called Opitz trigonocephaly-like syndrome. The C-like syndrome seems to be a severe form of the C syndrome. It is controversial whether there is (1) a gradient of spectrum in the C syndrome, from the mild form (C syndrome) to the severe form (C-like syndrome), or (2) genetic heterogeneity among the patients with the C syndrome.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Developmental stageExpressed at low levels on peripheral T-cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus.
- Information by UniProt
- CD96 antibody
- CD96 molecule antibody
- Cell surface antigen CD96 antibody
Formalin-fixed, paraffin-embedded MOLT-4 (human lymphoblastic leukemia cell line) cells stained for CD96 using ab264416 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
CD96 was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264416 at 6 µl per reaction. Western bot was performed on the immunoprecipitates using ab264416 at 1/1000 dilution.
Lane 1: ab264416 IP in MOLT-4 whole cell lysate.
Lane 2: Control IgG IP in MOLT-4 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
All lanes : Anti-CD96 antibody [BLR065G] - BSA free (ab264416) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : A2058 whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : CCRF-CEM (human T cell lymphoblast cell line) whole cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 8 : KG-1 whole cell lysate
Lane 9 : HH whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
ab264416 has not yet been referenced specifically in any publications.