Recombinant
RabMAb

Recombinant Anti-CD96 antibody [EPR22120-174] (ab224687)

Overview

  • Product name

    Anti-CD96 antibody [EPR22120-174]
    See all CD96 primary antibodies
  • Description

    Rabbit monoclonal [EPR22120-174] to CD96
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD96 aa 350-450. The exact sequence is proprietary.
    Database link: P40200

  • Positive control

    • ICC/IF: NK-92 cells. Flow: Human peripheral blood mononuclear cells; NK-92 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.
ICC/IF 1/50.
  • Application notes
    Is unsuitable for IHC-P,IP or WB.
  • Target

    • Function

      May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
    • Tissue specificity

      Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
    • Involvement in disease

      Defects in CD96 are a cause of C syndrome (CSYN) [MIM:211750]; also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. Note=A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint.
      Defects in CD96 are a cause of C-like syndrome (CLSYN) [MIM:605039]; also called Opitz trigonocephaly-like syndrome. The C-like syndrome seems to be a severe form of the C syndrome. It is controversial whether there is (1) a gradient of spectrum in the C syndrome, from the mild form (C syndrome) to the severe form (C-like syndrome), or (2) genetic heterogeneity among the patients with the C syndrome.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Developmental stage

      Expressed at low levels on peripheral T-cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD96 antibody
      • CD96 molecule antibody
      • Cell surface antigen CD96 antibody
      • DKFZp667E2122 antibody
      • MGC22596 antibody
      • T cell activated increased late expression protein antibody
      • T cell activation, increased late expression antibody
      • T cell surface protein tactile precursor antibody
      • T cell-activated increased late expression protein antibody
      • T-cell surface protein tactile antibody
      • TACT_HUMAN antibody
      • Tactile antibody
      see all

    Images

    • Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD96 with ab224687 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab224687 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. 

      Gated on viable cells.

    • Flow cytometric analysis of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling CD96 with ab224687 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling CD96 with ab224687 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in NK-92 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    References

    ab224687 has not yet been referenced specifically in any publications.

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