Product nameAnti-CD97 antibody [EPR4428]
See all CD97 primary antibodies
DescriptionRabbit monoclonal [EPR4428] to CD97
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to residues in Human CD97 (UniProt ID: P48960).
- U937 and THP-1 cell lysates, Human colonic adenocarcinoma tissue
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Tissue specificityBroadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain.
DomainThe first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain.
modificationsProteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- ADGRE5 antibody
- Adhesion G protein-coupled receptor E5 antibody
- CD 97 antibody
ab134105 has not yet been referenced specifically in any publications.