Overview

  • Product name

    Anti-CD97 antibody [EPR4428]
    See all CD97 primary antibodies
  • Description

    Rabbit monoclonal [EPR4428] to CD97
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CD97 (UniProt ID: P48960).

  • Positive control

    • U937 and THP-1 cell lysates, Human colonic adenocarcinoma tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
    • Tissue specificity

      Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 5 EGF-like domains.
      Contains 1 GPS domain.
    • Domain

      The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
      Binding to chondroitin sulfate is mediated by the fourth EGF domain.
    • Post-translational
      modifications

      Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
    • Cellular localization

      Cell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADGRE5 antibody
      • Adhesion G protein-coupled receptor E5 antibody
      • CD 97 antibody
      • CD_antigen=CD97 antibody
      • CD97 antibody
      • CD97 antigen antibody
      • CD97 antigen subunit beta antibody
      • CD97 molecule antibody
      • CD97_HUMAN antibody
      • Leukocyte antigen CD97 antibody
      • Seven span transmembrane protein antibody
      • Seven transmembrane helix receptor antibody
      • Seven transmembrane heterodimeric receptor associated with inflammation antibody
      • TM7LN 1 antibody
      • TM7LN1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.

    References

    ab134105 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134105.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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