Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4428] to CD97
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
Product nameAnti-CD97 antibody [EPR4428]
See all CD97 primary antibodies
DescriptionRabbit monoclonal [EPR4428] to CD97
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Rat, Human
corresponding to Human CD97 aa 700-800.
Database link: P48960
- WB: U937 and THP-1 cell lysates IHC-P: Human colonic adenocarcinoma tissue
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Tissue specificityBroadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain.
DomainThe first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain.
modificationsProteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- ADGRE5 antibody
- Adhesion G protein-coupled receptor E5 antibody
- CD 97 antibody
Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Lane 1 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/10000 dilution
Lane 2 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/20000 dilution
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Lane 1: 180 seconds
Lane 2: 60 seconds
The 28kDa is β-chain. This is supported by many papers, PMID: 20084281, PMID: 18941248.
ab134105 has not yet been referenced specifically in any publications.