FITC Anti-CD97 antibody [MEM-180] (ab23490)
Key features and details
- FITC Mouse monoclonal [MEM-180] to CD97
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
-
Product name
FITC Anti-CD97 antibody [MEM-180]
See all CD97 primary antibodies -
Description
FITC Mouse monoclonal [MEM-180] to CD97 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab23490 recognises the human CD97 cell surface antigen, a 74-89kD glycoprotein expressed by monocytes and activated lymphocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
PHA-activated PBL.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MEM-180 -
Isotype
IgG1 -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 20µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
Flow Cyt
Use 20µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. -
Tissue specificity
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain. -
Domain
The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain. -
Post-translational
modificationsProteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 976 Human
- Omim: 601211 Human
- SwissProt: P48960 Human
- Unigene: 466039 Human
-
Alternative names
- ADGRE5 antibody
- Adhesion G protein-coupled receptor E5 antibody
- CD 97 antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (2)
ab23490 has been referenced in 2 publications.
- Tian H et al. Effects of targeted CD97 immune epitopes small interference RNA on cellular biological behaviors in MDA-MB231 malignant breast cancer cell line. Am J Transl Res 9:4640-4651 (2017). PubMed: 29118924
- Ghazvini M et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS One 8:e73872 (2013). PubMed: 24069241