Overview

  • Product name
    Anti-CD98 antibody [44D7]
    See all CD98 primary antibodies
  • Description
    Mouse monoclonal [44D7] to CD98
  • Host species
    Mouse
  • Specificity
    The antibody reacts with the heavy chain of the CD98 molecule. It binds to all cell lines in culture and to activated T cells.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). HOON pre B leukaemia cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab23495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

Use 10µl of working dilution for 106 cells in 100µl.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.
  • Tissue specificity
    Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
  • Sequence similarities
    Belongs to the SLC3A transporter family.
  • Post-translational
    modifications
    Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
  • Cellular localization
    Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
  • Information by UniProt
  • Database links
  • Form
    There are 4 isoforms produced by alternative splicing.
  • Alternative names
    • 4F2 antibody
    • 4F2 cell surface antigen heavy chain antibody
    • 4F2 cell-surface antigen heavy chain antibody
    • 4F2 heavy chain antibody
    • 4F2 heavy chain antigen antibody
    • 4F2_HUMAN antibody
    • 4F2hc antibody
    • 4T2HC antibody
    • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
    • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
    • CD 98 antibody
    • CD98 antibody
    • CD98 antigen antibody
    • CD98 heavy chain antibody
    • CD98HC antibody
    • Heavy chain antibody
    • Lymphocyte activation antigen 4F2 large subunit antibody
    • MDU 1 antibody
    • MDU1 antibody
    • Monoclonal antibody 44D7 antibody
    • NACAE antibody
    • Slc3a2 antibody
    • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
    • Solute carrier family 3 member 2 antibody
    see all

Images

  • ab23495 staining CD98 in Human peripheral blood monocytes, detected with FITC-conjugated Rabbit anti-Mouse IgG as the secondary antibody.

References

This product has been referenced in:
  • Wu B  et al. Dominant Suppression of ß1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression. Int J Mol Sci 17:N/A (2016). WB, IHC, ICC/IF ; Human . Read more (PubMed: 27834933) »
  • Wu B  et al. Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma. J Exp Clin Cancer Res 34:110 (2015). Human . Read more (PubMed: 26437640) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and sorry for the delay of our reply.

Please find below the information I obtained for each anti-CD98 antibodies:

ab2528 : the epitope hasnot beenmapped.
ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



I would recommend the antibody https://www.abcam.com/ab108300.
To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

3. Test it in ICC/IF.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us with your question. We previously carried the non-conjugated CD98 antibody from the clone 4F2 as ab33232. The clone number for this antibody was changed by the production lab, and we now carry this antibody as ab23495 with the clone name "44D7". I can assure you that ab23495 is the same antibody as ab33232, and the clones 44D7 and 4F2 are the same clones. I hope that this information is helpful, but please let me know if you have any questions about this. I apologize for any confusion about the clone names.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton-X-100 in PBS
Blocking step
0,2 % Gelatine in PBS as blocking agent for 10 minute(s) · Concentration: 2000µg/mL · Temperature: 22°C

Dr. Dagmar Salber

Verified customer

Submitted Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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