Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)
![Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)](https://www.abcam.com/ps/products/23/ab23495/Images/ab23495-33345-anti-cd98-antibody-44d7-flow-cytometry.gif "Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)")
Key features and details
- Mouse monoclonal [44D7] to CD98 - Plasma Membrane Marker
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD98 antibody [44D7] - Plasma Membrane Marker
See all CD98 primary antibodies -
Description
Mouse monoclonal [44D7] to CD98 - Plasma Membrane Marker -
Host species
Mouse -
Specificity
The antibody reacts with the heavy chain of the CD98 molecule. It binds to all cell lines in culture and to activated T cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human). HOON pre B leukaemia cell line.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
The antibody was purified by affinity chromatography. -
Clonality
Monoclonal -
Clone number
44D7 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab23495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use 10µl of working dilution for 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. -
Tissue specificity
Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1. -
Sequence similarities
Belongs to the SLC3A transporter family. -
Post-translational
modificationsPhosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions. -
Cellular localization
Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 6520 Human
- Omim: 158070 Human
- SwissProt: P08195 Human
- Unigene: 502769 Human
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Form
There are 4 isoforms produced by alternative splicing. -
Alternative names
- 4F2 antibody
- 4F2 cell surface antigen heavy chain antibody
- 4F2 cell-surface antigen heavy chain antibody
see all
Images
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Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)ab23495 staining CD98 in Human peripheral blood monocytes, detected with FITC-conjugated Rabbit anti-Mouse IgG as the secondary antibody.
Protocols
References (3)
ab23495 has been referenced in 3 publications.
- Wu B et al. Dominant Suppression of ß1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression. Int J Mol Sci 17:N/A (2016). WB, IHC, ICC/IF ; Human . PubMed: 27834933
- Wu B et al. Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma. J Exp Clin Cancer Res 34:110 (2015). Human . PubMed: 26437640
- Letarte M et al. Correlations between the 44D7 antigenic complex and the plasma membrane Na+-Ca2+ exchanger. Biochem Cell Biol 64:1160-9 (1986). PubMed: 3828108
