Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)

Reviews (2)Q&A (2)References (5)
Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)

    Key features and details

    • Mouse monoclonal [44D7] to CD98 - Plasma Membrane Marker
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-CD98 antibody [44D7] - Plasma Membrane Marker
      See all CD98 primary antibodies

    • Description

      Mouse monoclonal [44D7] to CD98 - Plasma Membrane Marker

    • Host species

      Mouse

    • Specificity

      The antibody reacts with the heavy chain of the CD98 molecule. It binds to all cell lines in culture and to activated T cells.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human CD98. HOON pre B leukaemia cell line.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23495 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Use 10µl of working dilution for 106 cells in 100µl.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Use 10µl of working dilution for 106 cells in 100µl.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.

    • Tissue specificity

      Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.

    • Sequence similarities

      Belongs to the SLC3A transporter family.

    • Post-translational
      modifications

      Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.

    • Cellular localization

      Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
    • Information by UniProt

    • Database links

      • Form

        There are 4 isoforms produced by alternative splicing.

      • Alternative names

        • 4F2 antibody
        • 4F2 cell surface antigen heavy chain antibody
        • 4F2 cell-surface antigen heavy chain antibody
        • 4F2 heavy chain antibody
        • 4F2 heavy chain antigen antibody
        • 4F2_HUMAN antibody
        • 4F2hc antibody
        • 4T2HC antibody
        • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
        • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
        • CD 98 antibody
        • CD98 antibody
        • CD98 antigen antibody
        • CD98 heavy chain antibody
        • CD98HC antibody
        • Heavy chain antibody
        • Lymphocyte activation antigen 4F2 large subunit antibody
        • MDU 1 antibody
        • MDU1 antibody
        • Monoclonal antibody 44D7 antibody
        • NACAE antibody
        • Slc3a2 antibody
        • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
        • Solute carrier family 3 member 2 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)
        Flow Cytometry - Anti-CD98 antibody [44D7] - Plasma Membrane Marker (ab23495)
        ab23495 staining CD98 in Human peripheral blood monocytes, detected with FITC-conjugated Rabbit anti-Mouse IgG as the secondary antibody.

      References (5)

      Publishing research using ab23495? Please let us know so that we can cite the reference in this datasheet.

      ab23495 has been referenced in 5 publications.

      • Courtney J  et al. Abnormalities of placental development and function are associated with the different fetal growth patterns of hypoplastic left heart syndrome and transposition of the great arteries. Placenta 101:57-65 (2020). PubMed: 32927345
      • Gong S  et al. Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells. SLAS Discov 23:832-841 (2018). PubMed: 29505735
      • Wu B  et al. Dominant Suppression of ß1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression. Int J Mol Sci 17:N/A (2016). WB, IHC, ICC/IF ; Human . PubMed: 27834933
      • Wu B  et al. Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma. J Exp Clin Cancer Res 34:110 (2015). Human . PubMed: 26437640
      • Letarte M  et al. Correlations between the 44D7 antigenic complex and the plasma membrane Na+-Ca2+ exchanger. Biochem Cell Biol 64:1160-9 (1986). PubMed: 3828108

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-CD98 antibody [44D7] - Plasma Membrane Marker

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Melanoma cell line)
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Loading amount
      40 µg
      Specification
      Melanoma cell line
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 17 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD98 antibody [44D7]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa cells)
      Specification
      HeLa cells
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0,1% Triton-X-100 in PBS
      Blocking step
      0,2 % Gelatine in PBS as blocking agent for 10 minute(s) · Concentration: 2000µg/mL · Temperature: 22°C
      Read More

      Dr. Dagmar Salber

      Verified customer

      Submitted Feb 11 2008

      Question

      Inquiry: The CD98 protein is a heterodimer, composed of a heavy chain and a light chain linked by disulfide bonds. We're looking for an antibody staining ONLY the HEAVY CHAIN of CD98 (also named CD98hc, 4F2hc or SLC3A2) by immunofluorescence. The antibody clone MEM-108 is described as recognizing CD98, but it is not clear whether it would stains one or the other (or both) of the chains. The western-blot shown in the datasheet does not give a clear answer because the high molecular weigth signal suggests that the western-blot was done on a native gel (the expected size of the heterodimer is 125kDa whereas the heavy chain is approx. 80kDa and the light-chain 40kDa). Even if the heavy chain is highly glycosylated, we should expect a smaller band than what is shown on the figure... Do you have more info about the specificity of this antibody or about how this western-blot was performed ? Many thanks in advance for your help.

      Read More
      Answer

      Thank you for contacting us and sorry for the delay of our reply.

      Please find below the information I obtained for each anti-CD98 antibodies:

      ab2528 : the epitope hasnot beenmapped.
      ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
      ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



      I would recommend the antibody https://www.abcam.com/ab108300.
      To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

      2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

      3. Test it in ICC/IF.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

      Read More

      Question

      Dear Abcam, We had previously gotten a non-conjugated 4F2 anti-CD98 antibody from you. I see that you still have the 2 conjugated forms up the antibody on the website but not the unconjugated form. Is it still possible to get the unconjugated from you? Thanks,

      Read More
      Answer

      Thank you for contacting us with your question. We previously carried the non-conjugated CD98 antibody from the clone 4F2 as ab33232. The clone number for this antibody was changed by the production lab, and we now carry this antibody as ab23495 with the clone name "44D7". I can assure you that ab23495 is the same antibody as ab33232, and the clones 44D7 and 4F2 are the same clones. I hope that this information is helpful, but please let me know if you have any questions about this. I apologize for any confusion about the clone names.

      Read More

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