Recombinant
RabMAb

Recombinant Anti-CD98 antibody [EPR3548(2)] (ab108300)

Overview

  • Product name

    Anti-CD98 antibody [EPR3548(2)]
    See all CD98 primary antibodies
  • Description

    Rabbit monoclonal [EPR3548(2)] to CD98
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within the extracellular region of Human CD98

  • Positive control

    • HeLa, HepG2, Raji, and Molt-4 cell lysates, Human brain and Human testis tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.
    • Tissue specificity

      Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
    • Sequence similarities

      Belongs to the SLC3A transporter family.
    • Post-translational
      modifications

      Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
    • Cellular localization

      Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
    • Information by UniProt
    • Database links

    • Form

      There are 4 isoforms produced by alternative splicing.
    • Alternative names

      • 4F2 antibody
      • 4F2 cell surface antigen heavy chain antibody
      • 4F2 cell-surface antigen heavy chain antibody
      • 4F2 heavy chain antibody
      • 4F2 heavy chain antigen antibody
      • 4F2_HUMAN antibody
      • 4F2hc antibody
      • 4T2HC antibody
      • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
      • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
      • CD 98 antibody
      • CD98 antibody
      • CD98 antigen antibody
      • CD98 heavy chain antibody
      • CD98HC antibody
      • Heavy chain antibody
      • Lymphocyte activation antigen 4F2 large subunit antibody
      • MDU 1 antibody
      • MDU1 antibody
      • Monoclonal antibody 44D7 antibody
      • NACAE antibody
      • Slc3a2 antibody
      • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
      • Solute carrier family 3 member 2 antibody
      see all

    Images

    • All lanes : Anti-CD98 antibody [EPR3548(2)] (ab108300) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Molt-4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 68 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108300 at a dilution of 1/100.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108300 at a dilution of 1/100.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Robles-Valero J  et al. A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia. Cancer Cell 32:608-623.e9 (2017). Read more (PubMed: 29136506) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us and sorry for the delay of our reply.

    Please find below the information I obtained for each anti-CD98 antibodies:

    ab2528 : the epitope hasnot beenmapped.
    ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
    ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



    I would recommend the antibody https://www.abcam.com/ab108300.
    To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

    2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

    3. Test it in ICC/IF.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for providing this further information.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab108300 :

    - When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk as well as using the same blocking agent all along the protocol, not using milk for blocking and BSA for diluting the antibodies. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, it also helps in most cases to reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : www.abcam.com/ab9385.

    - I would also try an incubation with slightly more primary antibody, such as 1/500.

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

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