For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd99-antibody-12e7-ab8855.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity T Cells CD
Share by email

Anti-CD99 antibody [12E7] (ab8855)

  • Datasheet
  • SDS
Reviews (1)Q&A (8)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [12E7] to CD99
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

ELISA
Product image
Human Anti-Herpes simplex virus Type 1 IgG ELISA Kit (HSV1) (ab108737)
Primary
Product image
Anti-ALK antibody [SP8] (ab16670)
Primary
Product image
Anti-HNF-1B antibody [EPR18644-13] (ab213149)

View more associated products

Overview

  • Product name

    Anti-CD99 antibody [12E7]
    See all CD99 primary antibodies
  • Description

    Mouse monoclonal [12E7] to CD99
  • Host species

    Mouse
  • Specificity

    This antibody has been shown to give appropriate reactivity on a wide range of formalin-fixed, paraffin-embedded human tissue sections.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: Acute lymphocytic leukemia T-cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    12E7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • T Cell Adhesion
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD99 protein (His tag) (ab220556)

Target

  • Function

    Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similarities

    Belongs to the CD99 family.
  • Post-translational
    modifications

    Extensively O-glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P14209 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4267 Human
    • Omim: 313470 Human
    • SwissProt: P14209 Human
    • Unigene: 653349 Human
    • Alternative names

      • 12E7 antibody
      • Antigen identified by monoclonal 12E7, Y homolog antibody
      • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
      • CD99 antibody
      • CD99 antigen antibody
      • CD99 molecule antibody
      • CD99_HUMAN antibody
      • Cell surface antigen 12E7 antibody
      • Cell surface antigen HBA 71 antibody
      • Cell surface antigen O13 antibody
      • E2 antigen antibody
      • HBA71 antibody
      • MIC 2X antibody
      • MIC 2Y antibody
      • MIC2 (monoclonal antibody 12E7) antibody
      • MIC2 antibody
      • MIC2X antibody
      • MIC2Y antibody
      • MSK5X antibody
      • Protein MIC2 antibody
      • Surface antigen MIC2 antibody
      • T cell surface glycoprotein E2 antibody
      • T-cell surface glycoprotein E2 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab8855? Please let us know so that we can cite the reference in this datasheet.

    ab8855 has been referenced in 4 publications.

    • Rooper LM  et al. The Utility of NKX2.2 and TLE1 Immunohistochemistry in the Differentiation of Ewing Sarcoma and Synovial Sarcoma. Appl Immunohistochem Mol Morphol 27:174-179 (2019). PubMed: 28800015
    • Bode-Lesniewska B  et al. EWSR1-NFATC2 and FUS-NFATC2 Gene Fusion-Associated Mesenchymal Tumors: Clinicopathologic Correlation and Literature Review. Sarcoma 2019:9386390 (2019). PubMed: 31049020
    • Muff R  et al. Explant culture of sarcoma patients' tissue. Lab Invest 96:752-62 (2016). PubMed: 27111283
    • Hong SH  et al. Pharmacokinetic modeling optimizes inhibition of the 'undruggable' EWS-FLI1 transcription factor in Ewing Sarcoma. Oncotarget 5:338-50 (2014). Human . PubMed: 24481407

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Flow Cytometry abreview for Anti-CD99 antibody [12E7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Ewing Sarcoma Celline CHP)
    Specification
    Ewing Sarcoma Celline CHP
    Fixation
    No Fixation
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.