Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody is affinity purified.
Our Abpromise guarantee covers the use of ab27271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Staining pattern: Cell membrane
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
Sequence similaritiesBelongs to the CD99 family.
- Information by UniProt
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
Ab27271, prediluted, staining CD99 in paraffin embedded, formalin fixed human pancreas tissue by Immunohistochemistry.
All lanes : Anti-CD99 antibody (ab27271) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 19 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Cooper A et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res 17:56-66 (2011). IHC-P ; Human . Read more (PubMed: 21047978) »
- Makhoul R et al. Paranuclear dot-like immunostaining for CD99: presence in colonic adenomas and adenocarcinomas. Am J Surg Pathol 35:1749-50 (2011). Read more (PubMed: 21997696) »