Recombinant
RabMAb

Recombinant Anti-CD99 antibody [EPR3097Y] (ab75858)

Overview

  • Product name

    Anti-CD99 antibody [EPR3097Y]
    See all CD99 primary antibodies
  • Description

    Rabbit monoclonal [EPR3097Y] to CD99
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD99 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MOLT4, Jurkat and THP1 cell lysates; human Ewing's sarcoma and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab75858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
IP 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similarities

    Belongs to the CD99 family.
  • Post-translational
    modifications

    Extensively O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Images

  • Anti-CD99 antibody [EPR3097Y] (ab75858) at 1/1000 dilution (Purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • ab75858 at 1/1000 dilution staining CD99 in human Ewing's sarcoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry analysis of Jurkat ( Human acute T cell leukemia) labeling CD99 with purified ab75858 at 1/200 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde  and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • ab75858 at 1/1000 dilution staining CD99 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • All lanes : Anti-CD99 antibody [EPR3097Y] (ab75858) at 1/5000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Chung SS  et al. CD99 is a therapeutic target on disease stem cells in myeloid malignancies. Sci Transl Med 9:N/A (2017). WB ; Human . Read more (PubMed: 28123069) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

We currently do not have any product against the C.elegans protein MKS-5 nor will our products against a similar proteinRPGRIP1L recognize MKS-5. A protein with a similar name is MSK5X (also known as CD99). We do have a large selection of antibodies against this target. Ab8855 (https://www.abcam.com/CD99-antibody-12E7-ab8855.html) has been tested in ICC/IF while ab108297 (https://www.abcam.com/CD99-antibody-EPR3096-ab108297.html) and ab75858 (https://www.abcam.com/CD99-antibody-EPR3097Y-ab75858.html) are both guaranteed to work in ICH and ICC on human samples.

Abcam also carries a number of reagents for IF staining, including serums and blocking reagents, mounting media and background reducing buffers. OurBrightMount Plus Aqueous Mounting Medium for Fluorescent Staining (ab103748;https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html) is specifically designed for use in IF and would protect against photo-bleaching of your samples.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up