Recombinant Anti-CD99 antibody [SP119] (ab227738)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP119] to CD99
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD99 antibody [SP119]
See all CD99 primary antibodies -
Description
Rabbit monoclonal [SP119] to CD99 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD99.
Database link: P14209 -
Positive control
- IHC-P: Human pancreas, hepatocellular carcinoma, ovary, tonsil and thymus tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP119 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
Sequence similarities
Belongs to the CD99 family. -
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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Alternative names
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human ovary tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227738 has not yet been referenced specifically in any publications.