Overview

  • Product name

    Anti-CD99 antibody [SPM596] - BSA and Azide free
    See all CD99 primary antibodies
  • Description

    Mouse monoclonal [SPM596] to CD99 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD99 aa 1-185.
    Database link: P14209

  • Positive control

    • MOLT-4 cells. Pancreas or Ewing's sarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab212608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 min at Room Temp.

Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • Sequence similarities

    Belongs to the CD99 family.
  • Post-translational
    modifications

    Extensively O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ewing's sarcoma labeling CD99 with ab212608 at 2 µg/ml for 30 minutes at RT.

References

ab212608 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212608.
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