Anti-CD99L2 antibody (ab227075)

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Immunocytochemistry/ Immunofluorescence - Anti-CD99L2 antibody (ab227075)

    Key features and details

    • Rabbit polyclonal to CD99L2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-CD99L2 antibody
      See all CD99L2 primary antibodies

    • Description

      Rabbit polyclonal to CD99L2

    • Host species

      Rabbit

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rhesus monkey

    • Immunogen

      Recombinant fragment within Human CD99L2 (internal sequence). The exact sequence is proprietary.
      Database link: Q8TCZ2

    • Positive control

      • ICC/IF: A549 cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab227075 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100 - 1/1000.

    Target

    • Function

      May function as a homophilic adhesion molecule. Functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. Does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.

    • Tissue specificity

      Expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression seen in thymus. Expressed in E18 uterus and placenta.

    • Sequence similarities

      Belongs to the CD99 family.

    • Post-translational
      modifications

      O-glycosylated.

    • Cellular localization

      Cell membrane. Cell junction.
    • Information by UniProt

    • Database links

      • Alternative names

        • C99L2_HUMAN antibody
        • CD99 antibody
        • CD99 antigen-like protein 2 antibody
        • CD99L2 antibody
        • MIC2-like protein 1 antibody
        • MIC2L1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CD99L2 antibody (ab227075)
        Immunocytochemistry/ Immunofluorescence - Anti-CD99L2 antibody (ab227075)

        Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD99L2 (green) using ab227075 at 1/500 dilution in ICC/IF.

        Blue: Hoechst 33342 staining.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab227075? Please let us know so that we can cite the reference in this datasheet.

      ab227075 has not yet been referenced specifically in any publications.

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