Anti-CDA antibody (ab137605)
Key features and details
- Rabbit polyclonal to CDA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CDA antibody
See all CDA primary antibodies -
Description
Rabbit polyclonal to CDA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human CDA aa 1-146.
Database link: P32320 -
Positive control
- WB: A431, HeLa whole cell extracts. Mouse kidney lysate. IHC-P: Gastric CA N87 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Relevance
CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes. -
Database links
- Entrez Gene: 616377 Cow
- Entrez Gene: 978 Human
- Entrez Gene: 72269 Mouse
- Entrez Gene: 362638 Rat
- Omim: 123920 Human
- SwissProt: P32320 Human
- SwissProt: P56389 Mouse
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Alternative names
- CDD antibody
- Cytidine aminohydrolase antibody
- Cytidine deaminase antibody
- EC 3.5.4.5 antibody
Images
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Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft labelling CDA with ab137605 at 1/100 dilution.
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Anti-CDA antibody (ab137605) at 1/500 dilution + Mouse kidney lysate at 50 µg
Predicted band size: 16 kDa -
All lanes : Anti-CDA antibody (ab137605) at 1/1000 dilution
Lane 1 : A431 whole cell extracts
Lane 2 : HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137605 has been referenced in 2 publications.
- Aughton K et al. hENT1 Predicts Benefit from Gemcitabine in Pancreatic Cancer but Only with Low CDA mRNA. Cancers (Basel) 13:N/A (2021). PubMed: 34830914
- Céspedes MV et al. Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models. Dis Model Mech 9:1461-1471 (2016). IHC-P . PubMed: 27780828