Overview

  • Product name

  • Description

    Rabbit polyclonal to CDA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Recombinant full length protein (His-T7-tag) corresponding to Human CDA aa 2-146. Expresssed in E.coli.
    Sequence:

    AQKRPACTLKPECVQQLLVCSQEAKKSAYCPYSHFPVGAALLTQEGRIFK GCNIENACYPLGICAERTAIQKAVSEGYKDFRAIAIASDMQDDFISPCGA CRQVMREFGTNWPVYMTKPDGTYIVMTVQELLPSSFGPEDLQKTQ


    Database link: P32320

  • Positive control

    • WB: Recombinant human CDA protein; HeLa whole cell lysate; Pig kidney tissue lysate. IHC-P: Human kidney, lung cancer, skin cancer and bile duct cancer tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 20 µg/ml.
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 16 kDa.

Target

  • Relevance

    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.
  • Database links

  • Alternative names

    • CDD antibody
    • Cytidine aminohydrolase antibody
    • Cytidine deaminase antibody
    • EC 3.5.4.5 antibody

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CDA using ab231981 at 10 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDA using ab231981 at 20μg/ml in ICC/IF.

  • Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.

  • Anti-CDA antibody (ab231981) at 3 µg/ml + Recombinant human CDA

    Secondary
    HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

  • All lanes : Anti-CDA antibody (ab231981) at 3 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Pig kidney lysate

    Secondary
    All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

References

ab231981 has not yet been referenced specifically in any publications.

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