Overview

  • Product name
  • Description
    Rabbit polyclonal to CDA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to N terminal residues of Human CDA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.
  • Database links
  • Alternative names
    • CDD antibody
    • Cytidine aminohydrolase antibody
    • Cytidine deaminase antibody
    • EC 3.5.4.5 antibody

References

This product has been referenced in:
  • Bou Samra E  et al. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nat Commun 8:693 (2017). Read more (PubMed: 28947735) »
  • Gemble S  et al. Cytidine deaminase deficiency impairs sister chromatid disjunction by decreasing PARP-1 activity. Cell Cycle 16:1128-1135 (2017). Read more (PubMed: 28463527) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful so far. However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.
The details provided in the questionnaire enable us to closely monitor the quality of our products.
As requested, I have issued a free of charge replacement
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Question

Following our telephone conversation regarding the cda and dck antibody that we have recently ordered from abcam, I am attaching both the questionnaire and a PowerPoint slide containing the result of the blots.

In substitution of the antibodies that we have ordered we would like to try those two other clones:

Cda ab82346

Dck ab96599




Abcam product code ab




Anti CDA antibody Ab56053

Anti DCK antibody Ab38012




Abcam order reference number or product batch number



Description of the problem




I get a lot of background of unspecific bands that unable to let me predict which is the band of interest



4) Sample preparation:

Type of sample: cell lysates

Lysis buffer : CELL LYTIC M CELL LYSIS REAGENT

Protease inhibitors: PROTEASE INHIBITOR COCKTAIL SIGMA

Phosphatase inhibitors : PHOSPHATASE INHIBITOR ACTIVE MOTIF

Reducing agent N/A

Boiling for ≥5 min? yes

Protein loaded ug/lane or cells/lane : 30ug per well

Positive control : Calnexin

Negative control N/A



5) Percentage of gel: 7% or 4-12% precast gel

Type of membrane IMMOBILON V TRANSFER MEMBRANE

Protein transfer verified yes

Blocking agent and concentration 5%BSA in TBS-T

Blocking time 1h

Blocking temperature room temperature



6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

1:1000

Diluent buffer 5%BSA IN TBS-T

Incubation time OVERNIGHT

Incubation temperature: AT 4 DEGREES



7) Secondary antibody:

Species: ANTIRABBIT

Reacts against: HUMAN

Concentration or dilution 1:1000

Diluent buffer 5% BSA IN TBS-T

Incubation time 1H

Incubation temperature: ROOM TEMPERATURE

Fluorochrome or enzyme conjugate: ECL



8) Washing after primary and secondary antibodies:

Buffer (TBST-TWEEN)

Number of washes 3 WASHES



9)Detection method ECL

10) How many times have you run this staining? AT LEAST 3

Do you obtain the same results every time? YES

What steps have you altered to try and optimize the use of this antibody?

I TRIED TO BLOCK IN MILK

I USED 2 DIFFERENT DILUTIONS FOR DCK AND CDA: 1: 1000 and 1:500

Read More
Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.
Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab38012 and ab56053. I would also appreciate if you can confirm some further details:
Overall, your protocol looks all right. However, the secondary concentration is unusual high. Have you considered a non-primary control? In addition, I would advise to try the antibody in a much lower dilution, at least 1:10000.
Another point is the specificity of the secondary antibody, which is a bit confusing: Does this antibody react against human?

Should the suggestions not improve the results, please do let me know.
In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

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Answer

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
As we discussed on the phone, I am our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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