Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionMight be involved in nuclear membrane integrity.
Tissue specificityUbiquitously expressed. Isoform 3 is not found in erythroid cells.
Involvement in diseaseDefects in CDAN1 are the cause of congenital dyserythropoietic anemia type 1 (CDA1) [MIM:224120]. An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.
- Information by UniProt
- CDA1 antibody
- CDAI antibody
- CDAN1 antibody
All lanes : Anti-CDAN1 antibody (ab31236) at 0.5 µg/ml
Lane 1 : Hela B Cell Lysate at 30 µg
Lane 2 : Hela B Cell Lysate at 60 µg
Predicted band size: 134 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa (possible glycosylated form), 170 kDa (possible non-specific binding)
CDAN1 antibody ab31236 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to CDAN1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-CDAN1 antibody (ab31236) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 20 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 145 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab31236 has not yet been referenced specifically in any publications.