The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
Use a concentration of 1 µg/ml.
Might be involved in nuclear membrane integrity.
Ubiquitously expressed. Isoform 3 is not found in erythroid cells.
Involvement in disease
Defects in CDAN1 are the cause of congenital dyserythropoietic anemia type 1 (CDA1) [MIM:224120]. An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.
CDAN1 antibody ab31236 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to CDAN1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-CDAN1 antibody (ab31236)
Anti-CDAN1 antibody (ab31236) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 20 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 134 kDa Observed band size: 134 kDa Additional bands at: 145 kDa, 70 kDa. We are unsure as to the identity of these extra bands.