Product nameAnti-CDC123 antibody
See all CDC123 primary antibodies
DescriptionRabbit polyclonal to CDC123
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human CDC123 aa 275-325. The exact sequence is proprietary.
Database link: O75794
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226369 was affinity purified using an epitope specific to CDC123 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 39 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for S phase entry of the cell cycle.
Tissue specificityWidely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis.
Sequence similaritiesBelongs to the CDC123 family.
- Information by UniProt
- C10orf7 antibody
- CD123_HUMAN antibody
- CDC123 antibody
All lanes : Anti-CDC123 antibody (ab226369) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 30 seconds
Cell lysates were prepared with NETN lysis buffer.
CDC123 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226369 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226369 at 1 µg/ml.
Lane 1: ab226369 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226369 has not yet been referenced specifically in any publications.